ABSTRACT: The accumulation of organic matter (OM) in the sediment of aquaculture ponds is a potential threat for aquaculture ecosystems and the surrounding environment. The snail Bellamya purificata is a potential bioremediation species which may solve this problem. To investigate the effects of B. purificata on OM degradation in surface sediment, an 84 d experiment was carried out. The experimental setup entailed 6 glass tanks which were divided into B. purificata treatment (BPT) and control (CON). At the end of the experiment, a significantly lower degradation index (DI), reactivity index (RI), and carbon-normalized yield of amino acids were observed in BPT compared to CON, with mean ± SD values of -0.47 ± 0.43, 1.24 ± 0.01, and 6.24 ± 0.44, respectively. BPT showed higher oxidation-reduction potential and bacterial 16S rRNA gene copies than CON. Total organic carbon, total nitrogen, and total hydrolysable amino acid concentrations in the BPT treatment were 1.83 ± 0.10%, 0.07 ± 0.01%, and 22.38 ± 0.53 µmol g^-1, respectively, all of which were significantly lower than in CON. A clustered heat-map of different indexes related to OM degradation in sediments showed the final BPT as one separate category, which was different from the initial samples and the final CON. Overall, B. purificata could effectively facilitate OM degradation by promoting oxidation-reduction potential and bacterial populations, and ultimately by inhibiting the OM accumulation in sediment. Our results therefore provide support for the application of B. purificata to reduce the risk of endogenous pollution caused by OM accumulation in aquaculture ponds.

中文翻译：

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氨基酸揭示的风铃草对表层沉积物中有机物降解的影响

摘要：水产养殖池塘沉积物中有机物的积累是对水产养殖生态系统和周围环境的潜在威胁。蜗牛贝拉米亚purificata是一种潜在的生物修复物种，可以解决这个问题。为了研究纯化芽孢杆菌对表面沉积物中OM降解的影响，进行了84天的实验。实验设置需要将6个玻璃罐分成B. purificata治疗（BPT）和对照（CON）。在实验结束时，与CON相比，BPT中观察到的降解指数（DI），反应性指数（RI）和氨基酸的碳归一化产率显着降低，平均值±SD值为-0.47±0.43，1.24分别为±0.01和6.24±0.44。BPT显示出比CON高的氧化还原电位和细菌16S rRNA基因拷贝。BPT处理中的总有机碳，总氮和总可水解氨基酸浓度为1.83±0.10％，0.07±0.01％和22.38±0.53 µmol g ^-1，所有这些都显着低于CON。与沉积物中OM降解相关的不同指标的聚类热图显示，最终的BPT是一个单独的类别，与初始样品和最终的CON不同。总体而言，B。purificata可以通过促进氧化还原电位和细菌种群，并最终通过抑制OM在沉积物中的积累，有效地促进OM降解。因此，我们的结果为应用B. purificata降低水产养殖池塘中OM积累引起的内源性污染的风险提供了支持。