Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is a common disease of soybean (Glycine max (L.) Merr.) worldwide, seriously affecting yield. Kangxian 2 is a soybean variety with a high level of resistance to H. glycines (HG) Type 0 (SCN race 3) and a yellow seed coat. However, we know little about the mechanism of resistance to HG Type 0 in Kangxian 2. In this study, we used the Illumina HiSeq high-throughput sequencing platform to analyse the transcriptome of Kangxian 2 and obtained 65.74 Gb clean data. Transcriptional changes in Kangxian 2 caused by HG Type 0 stress after 0–10 days are described. Kangxian 2 showed different levels of gene expression after inoculation, and under HG Type 0 stress after different times. Overall, 6854 HG Type 0-induced genes and 5328 HG Type 0-repressed genes were found to be differentially regulated. The greatest number of differential genes annotated to cellular process, metabolic process, single-organism process, binding, catalytic activity and other pathways. In addition to findings of differentially expressed genes similar to other published work, such as the regulation of biosynthesis of many secondary metabolites, carbon sequestration of photosynthetic organisms, other types of O-polysaccharide biosynthesis, phenylpropane biosynthesis, pyruvate metabolism and other pathways, this study also revealed the differential regulation of genes related to the diarylheptanoid and gingerol biosynthesis pathway and found some metabolic pathways that were specifically expressed in the syncytial initiation and establishment stages. Gene expression analyses using real-time fluorescence quantitative PCR showed that the expression of GmMADS and GmTUB changed strongly after 7 days and 10 days of HG Type 0 stress compared with the control. We conclude that GmMADS and GmTUB transcription factor genes may play an important role in the resistance of Kangxian 2 to HG Type 0 stress.

大豆囊肿线虫（SCN，异戊三烯甘氨酸Ichinohe）是世界范围内常见的大豆疾病（大豆Glycine max（L.）Merr。），严重影响产量。康县2号是对甘氨酸高抗性的大豆品种（HG）0型（SCN第3种族）和黄色种皮。但是，我们对抗氧剂2中HG型0的抗性机理了解甚少。在这项研究中，我们使用Illumina HiSeq高通量测序平台分析抗氧剂2的转录组，并获得65.74 Gb的干净数据。描述了0-10天后由0型HG引起的抗县2号转录变化。康县2号接种后表现出不同水平的基因表达，不同时期在HG 0型胁迫下表现出不同水平。总体而言，发现6854 HG 0型诱导基因和5328 HG 0型抑制基因受到差异调节。注释最多的差异基因包括细胞过程，代谢过程，单一生物过程，结合，催化活性和其他途径。O-多糖的生物合成，苯丙烷的生物合成，丙酮酸的代谢等途径，这项研究还揭示了与二芳基庚烷和姜醇生物合成途径有关的基因的差异调控，并发现了一些在合胞体起始和建立阶段特异性表达的代谢途径。使用实时荧光定量PCR进行的基因表达分析表明，与对照组相比，HG 0型应激7天和10天后GmMADS和GmTUB的表达发生了强烈变化。我们得出的结论是，GmMADS和GmTUB转录因子基因可能在抗2号抗HG型0胁迫中起重要作用。