The role of systemic inflammation in cancer's progression has been widely investigated, especially in melanoma in humans. Pre‐treatment leukocyte counts and ratios play a recognized prognostic role in several types of malignancies, but no information is available regarding canine oral malignant melanoma (COMM). The purpose of this explorative retrospective study was to investigate the prognostic impact of pre‐treatment neutrophil to lymphocyte (NLR) and lymphocyte to monocyte (LMR) ratios in dogs with oral malignant melanoma that underwent surgical resection and immunotherapy with adjuvant CSPG4‐antigen electrovaccination. Thirty‐nine dogs with histologically confirmed oral melanoma and with available pre‐treatment haematological analyses, performed at maximum 60 days before the first treatment, were retrospectively enrolled. Statistical analysis was performed to explore possible correlations among NLR and LMR with age, clinical stage, tumour pigmentation, tumour size, nuclear atypia, mitotic index, Ki67, CSPG4 expression, ulceration, bone invasion and excision margins status. The impact of NLR and LMR on overall survival time (OST) was explored among various ratio cut off and across different time points with Kaplan‐Meier method. No significant relationship was identified between leukocytes ratios and histological parameters, CSPG4 expression, excision margin status, age, tumour size and clinical stage. NLR and LMR did not display a prognostic impact on the survival time of the entire population. Pre‐treatment leukocyte ratios may not represent a useful prognostic factor in dogs with oral melanoma, especially in absence of distant metastatic disease.

中文翻译：

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评估预处理中性粒细胞与淋巴细胞和淋巴细胞与单核细胞比率对接受手术和辅助 CSPG4 抗原电接种治疗的口腔恶性黑色素瘤犬的预后影响：一项探索性研究

全身炎症在癌症进展中的作用已被广泛研究，尤其是在人类黑色素瘤中。治疗前白细胞计数和比率在几种类型的恶性肿瘤中发挥公认的预后作用，但没有关于犬口腔恶性黑色素瘤 (COMM) 的信息。这项探索性回顾性研究的目的是研究预处理中性粒细胞与淋巴细胞 (NLR) 和淋巴细胞与单核细胞 (LMR) 比率对接受手术切除和辅助 CSPG4 抗原电疫苗免疫治疗的口腔恶性黑色素瘤犬的预后影响。回顾性招募了 39 只经组织学证实为口腔黑色素瘤并进行了治疗前血液学分析的狗，这些狗在第一次治疗前最多 60 天进行。进行统计分析以探索 NLR 和 LMR 与年龄、临床分期、肿瘤色素沉着、肿瘤大小、核异型性、有丝分裂指数、Ki67、CSPG4 表达、溃疡、骨侵袭和切除边缘状态的可能相关性。使用 Kaplan-Meier 方法探讨了不同比率截止值和不同时间点之间 NLR 和 LMR 对总生存时间 (OST) 的影响。在白细胞比率和组织学参数、CSPG4 表达、切除边缘状态、年龄、肿瘤大小和临床分期之间没有发现显着关系。NLR 和 LMR 对整个人群的生存时间没有显示出预后影响。治疗前白细胞比率可能不代表患有口腔黑色素瘤的狗的有用预后因素，尤其是在没有远处转移性疾病的情况下。