GNOM‐dependent endocytosis maintains polar localization of the borate exporter BOR1 in Arabidopsis,Biology of the Cell

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GNOM‐dependent endocytosis maintains polar localization of the borate exporter BOR1 in Arabidopsis
Biology of the Cell ( IF 2.4 ) Pub Date : 2021-02-03 , DOI: 10.1111/boc.202000106
Akira Yoshinari _{1,

2} , Yosuke Toda _{1,

3,

4} , Junpei Takano ₂

Affiliation

BACKGROUND INFORMATION Plants use transporters polarly localized in the plasma membrane for the directional transport of nutrients. The boric acid/borate (B) exporter BOR1 is localized polarly in the inner lateral domain of the plasma membrane in various root cells for efficient translocation of B under B limitation. With a high B supply, BOR1 is ubiquitinated and transported to vacuoles for degradation. The polar localization and vacuolar targeting of BOR1 are maintained by different endocytosis mechanisms. RESULTS We demonstrated that one of the most utilized inhibitors in endosomal recycling, brefeldin A (BFA), inhibits the polar localization of BOR1. BFA inhibits a subset of guanine-nucleotide exchange factors (ARF-GEFs), regulators of vesicle formation. Using a transgenic line expressing BFA-resistant engineered GNOM, we identified GNOM as the key ARF-GEF in endocytosis and maintenance of the polar localization of BOR1. CONCLUSIONS AND SIGNIFICANCE We found that BFA inhibits the polar localization of BOR1 by inhibiting GNOM activity. Our results suggest that GNOM-dependent endocytosis contributes to the maintenance of the polar localization of BOR1 under B limitation. We propose a model of BOR1 transcytosis initiated from GNOM-dependent endocytosis. This article is protected by copyright. All rights reserved.

中文翻译：

GNOM 依赖性内吞作用维持拟南芥中硼酸盐输出蛋白 BOR1 的极性定位

背景信息植物使用极性定位在质膜中的转运蛋白来定向运输养分。硼酸/硼酸盐 (B) 出口蛋白 BOR1 极性定位于各种根细胞质膜的内侧域，以便在 B 限制下有效转移 B。在高 B 供应的情况下，BOR1 被泛素化并运输到液泡进行降解。BOR1 的极性定位和液泡靶向由不同的内吞机制维持。结果我们证明了在内体循环中最常用的抑制剂之一，brefeldin A (BFA) 抑制 BOR1 的极性定位。BFA 抑制鸟嘌呤核苷酸交换因子 (ARF-GEF) 的子集，即囊泡形成的调节剂。使用表达 BFA 抗性工程 GNOM 的转基因品系，我们将 GNOM 确定为内吞作用和维持 BOR1 极性定位的关键 ARF-GEF。结论和意义我们发现 BFA 通过抑制 GNOM 活性来抑制 BOR1 的极性定位。我们的结果表明依赖于 GNOM 的内吞作用有助于在 B 限制下维持 BOR1 的极性定位。我们提出了一种从依赖于 GNOM 的内吞作用开始的 BOR1 转胞吞作用模型。本文受版权保护。版权所有。我们提出了一种从依赖于 GNOM 的内吞作用开始的 BOR1 转胞吞作用模型。本文受版权保护。版权所有。我们提出了一种从依赖于 GNOM 的内吞作用开始的 BOR1 转胞吞作用模型。本文受版权保护。版权所有。

更新日期：2021-02-03

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