Theoretical and experimental studies were conducted to determine the role of 1-MCP in the biosynthesis of ethylene in cape gooseberry fruits (Physalis peruviana L.), ecotype Colombia. A completely randomized experimental design was used with 3 treatments: ethylene (1000 μL ethephon L⁻¹), 1-MCP (1 μL L⁻¹), and a control with no applications. At 1, 6 and 11 days after treatment, volatile compounds, firmness and color were measured. The enzymatic activities of ACC oxidase (E.C. 1.14.17.4) and histidine kinase (HK, E.C. 2.7.13.3) were also determined in vitro. Subsequently, molecular docking studies with the enzymes, their respective substrates and 1-MCP were carried out. It was found that 1-MCP decreased the emission of volatile esters, color index, loss of firmness, and ACC oxidase and histidine kinase activities. Results indicated 1-MCP acted as a competitive inhibitor of ACC oxidase and as a noncompetitive inhibitor of HK. The coupling free energy was higher for 1-MCP in both the ACC oxidase (8.31) and the HK (4.22), compared to their respective substrates. The results also suggested that 1-MCP was able to decrease both the biosynthesis and activity of ethylene in cape gooseberry fruits.

进行了理论和实验研究，以确定1-MCP在哥伦比亚生态型灯笼果（Physalis peruviana L.）中乙烯生物合成中的作用。使用3种处理方法的完全随机实验设计：乙烯（1000μL乙烯利L ^-1），1-MCP（1μLL ^-1）和无应用的对照。处理后第1、6和11天，测量挥发性化合物，硬度和颜色。ACC氧化酶的酶活性（EC 1.14.17.4）和组氨酸激酶（HK，EC 2.7.13.3）还测定在体外。随后，进行了酶，它们各自的底物和1-MCP的分子对接研究。发现1-MCP降低了挥发性酯的释放，颜色指数，硬度的损失以及ACC氧化酶和组氨酸激酶活性。结果表明1-MCP充当ACC氧化酶的竞争性抑制剂和HK的非竞争性抑制剂。与它们各自的底物相比，ACC氧化酶（8.31）和HK（4.22）中1-MCP的偶合自由能更高。该结果还表明1-MCP能够降低灯笼果果实中乙烯的生物合成和活性。