Induced pluripotent stem cells (iPSCs) are considered to be ideal and promising cell sources for various applications such as regenerative medicine and drug screening. However, effective mass production systems for the stable supply of desired numbers of iPSCs are yet to be developed. This review introduces the various approaches that are currently available for stable iPSC production. We start by discussing the limiting factors to be controlled during iPSC culture, such as nutrient supply, waste removal, and oxygen availability. We then introduce recent investigations on iPSC culture systems based on adhesion, suspension, and scaffolds. We also discuss the downstream processes that follow the culture process, such as filling and freezing processes, which limit the production scale due to decreased cell viability during suspension in cryopreservation medium. Finally, we summarize the possibility of the stable mass production of iPSCs and highlight the limitations that remain to be overcome. We suggest that multidisciplinary investigations are essential to understand the different factors that influence cell growth and quality in order to obtain an optimal and stable iPSC mass production system.

诱导多能干细胞（iPSC）被认为是用于各种应用（例如再生医学和药物筛选）的理想且有希望的细胞来源。但是，尚未开发出用于稳定供应所需数量的iPSC的有效批量生产系统。这篇评论介绍了目前可用于稳定iPSC生产的各种方法。我们首先讨论在iPSC培养过程中要控制的限制因素，例如营养供应，废物清除和氧气供应。然后，我们介绍基于粘附，悬浮和支架的iPSC培养系统的最新研究。我们还讨论了培养过程之后的下游过程，例如灌装和冷冻过程，由于在冷冻保存介质中悬浮过程中细胞活力降低，从而限制了生产规模。最后，我们总结了稳定批量生产iPSC的可能性，并强调了仍有待克服的局限性。我们建议多学科研究对于了解影响细胞生长和质量的不同因素至关重要，以便获得最佳且稳定的iPSC批量生产系统。