Mechanobiology is focused on how the physical forces and mechanical properties of proteins, cells and tissues contribute to physiology and disease. While the response of proteins and cells to mechanical stimuli is critical for function, the tools to probe these activities are typically restricted to single molecule manipulations. Here, we have developed a novel microplate reader assay to encompass mechanical measurements with ensemble biochemical and cellular assays, using a microplate lid modified with magnets. This configuration enables multiple static magnetic tweezers to function simultaneously across the microplate, thereby greatly increasing throughput. We demonstrate the broad applicability and versatility through in vitro and in cellulo approaches. Overall, our methodology allows, for the first-time, ensemble biochemical and cell-based assays to be performed under force, in high throughput format. This approach substantially increases the availability of mechanobiology measurements.

中文翻译：

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高通量机械生物学：整体生化和细胞检测的力调节


力学生物学专注于蛋白质、细胞和组织的物理力和机械特性如何影响生理和疾病。虽然蛋白质和细胞对机械刺激的反应对于功能至关重要，但探测这些活动的工具通常仅限于单分子操作。在这里，我们开发了一种新颖的酶标仪测定，使用经过磁铁修饰的微孔板盖，涵盖机械测量以及整体生化和细胞测定。这种配置使多个静磁镊子能够在微孔板上同时工作，从而大大提高通量。我们通过体外和细胞方法证明了广泛的适用性和多功能性。总体而言，我们的方法首次允许以高通量形式在强制下进行整体生化和基于细胞的测定。这种方法大大提高了力学生物学测量的可用性。