A feeding trial was carried out to investigate the effects of dietary fermented rice protein (FRP) on growth, digestive and absorption capacities, inflammatory-related gene expression, and intestinal microbiota in juvenile hybrid groupers (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂). A fish meal (FM)-based diet was used as a control for comparison with diets in which FM was replaced by FRP at percentages of 10 % (FRP10), 30 % (FRP30), and 50 % (FRP50). Each diet was fed to triplicate groups of fish for 56 days. We observed no significant differences in weight gain rate, specific growth rate, and protein productive value between the FM and FRP10 groups, but significantly lower rates in the FRP30 and FRP50 groups. The activities of the intestinal digestive enzymes (amylase, trypsin, and pepsin) and brush border enzymes (Na⁺/K⁺-ATPase, creatine kinase, and γ-glutamyl transpeptidase) were higher in the FRP-containing groups than in the FM group. The expression levels of immune-related genes (TLR22, MyD88, TNF-α, IL-2, IL-6, IL-8, and TGF-β1) were all down-regulated in FRP-fed fish. Intestinal microbiota showed no significant differences in alpha diversity among dietary groups. At the genus level, FRP-containing groups had lower relative abundances of Bacteroides and Enterococcus than the FM group. In conclusion, 10 % of the FM protein in the feed can be replaced by FRP without adverse effects on nutritional status. Replacing FM with FRP in the feed suppresses inflammatory function in hybrid groupers. It also has a critical influence on microbial profiles but not on diversity.

进行了一项喂养试验，以研究日粮发酵稻米蛋白（FRP）对少年杂种石斑鱼（Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus）生长，消化和吸收能力，炎症相关基因表达以及肠道菌群的影响♂）。以鱼粉（FM）为基础的饮食用作对照，与以10％（FRP10），30％（FRP30）和50％（FRP50）的百分比用FRP代替FM的饮食进行比较。每种饮食喂给三组鱼共56天。我们观察到FM和FRP10组之间在体重增加率，比生长率和蛋白质生产价值上没有显着差异，但是在FRP30和FRP50组中比率显着降低。肠内消化酶（淀粉酶，胰蛋白酶和胃蛋白酶）和刷缘酶（Na ⁺ / K ⁺-ATPase，肌酸激酶和γ-谷氨酰转肽酶在含FRP的组中高于FM组。在FRP喂养的鱼中，免疫相关基因（TLR22，MyD88，TNF-α，IL-2，IL-6，IL-8和TGF-β1）的表达水平均下调。饮食组之间的肠道菌群无明显差异。在属水平上，含FRP的组的拟杆菌和肠球菌的相对丰度低于FM组。总之，饲料中10％的FM蛋白可以被FRP替代，而对营养状况没有不利影响。饲料中用FRP代替FM可抑制杂种石斑鱼的炎症功能。它也对微生物谱有关键影响，但对多样性没有影响。