Chytridiomycosis is a fungal disease caused by the pathogens, Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), which has caused declines in amphibian populations worldwide. Asia is considered as a coldspot of infection, since adult frogs are less susceptible to Bd-induced mortality or morbidity. Using the next-generation sequencing approach, we assessed the cutaneous bacterial community composition and presence of anti-Bd bacteria in six frog species from India using DNA isolated from skin swabs. All the six frog species sampled were tested using nested PCR and found Bd negative. We found a total of 551 OTUs on frog skin, of which the bacterial phyla such as Proteobacteria (56.15% average relative abundance) was dominated followed by Actinobacteria (21.98% average relative abundance) and Firmicutes (13.7% average relative abundance). The contribution of Proteobacteria in the anti-Bd community was highest and represented by 175 OTUs. Overall, the anti-Bd bacterial community dominated (51.7% anti-Bd OTUs) the skin microbiome of the frogs. The study highlights the putative role of frog skin microbiome in affording resistance to Bd infections in coldspots of infection.

壶菌病是一种真菌病害，由病原体树突杆菌( Bd ) 和双歧杆菌( Bsal ) 引起，已导致全球两栖动物种群数量下降。亚洲被认为是感染的冷点，因为成年青蛙不太容易受到Bd引起的死亡率或发病率的影响。使用下一代测序方法，我们使用从皮肤拭子中分离的 DNA 评估了来自印度的六种青蛙的皮肤细菌群落组成和抗Bd细菌的存在。使用巢式 PCR 对所有六种蛙类采样进行了测试，发现Bd消极的。我们在青蛙皮肤上共发现了 551 个 OTU，其中以变形杆菌门（平均相对丰度为 56.15%）为主，其次是放线菌门（平均相对丰度为 21.98%）和厚壁菌门（平均相对丰度为 13.7%）。Proteobacteria 在抗Bd群落中的贡献最高，由 175 个 OTU 代表。总体而言，抗Bd细菌群落在青蛙的皮肤微生物组中占主导地位（51.7% 的抗Bd OTU）。该研究强调了青蛙皮肤微生物组在抵抗感染冷点的Bd感染方面的推定作用。