Objective: The aim of this study was to compare muscle oxygenation of the vastus lateralis during a high volume, isokinetic resistance exercise protocol (HVP) between young adult (YA) and middle-aged adult (MA) men.Methods: Twenty recreationally trained men were assigned to either the YA (21.8±2.0 y, 90.7±11.6 kg, 179±4.7 cm) or MA (47.0±4.4 y, 96.1±21.6 kg, 177±7.7 cm) group. The HVP consisted of eight sets of 10 repetitions of unilateral isokinetic concentric knee extension and eccentric knee flexion at 60°·sec-1. Changes in tissue hemoglobin saturation index (TSI), tissue oxygenated hemoglobin concentration (O2Hb), deoxygenated hemoglobin (HHb), and muscle oxidation index (O2Hb-HHb) were measured during the exercise session using near-infrared spectroscopy (NIRS). Data were analyzed using two-way mix factorial analyses of variance. Results: Prior to exercise, TSI was significantly greater for YA compared to MA (73.2±3.4% vs. 68.6±4.8%, p=0.024). For both groups, significant decreases in O2Hb and O2Hb-HHb, and increases in HHb were observed during each of the eight sets relative to the rest periods (p<0.05). However, the average change during the eight sets of the HVP revealed a significantly higher (p=0.036) level of HHb (7.8±0.5μM vs. 5.3±3.1μM) and a lower (p=0.029) level of O2Hb-HHb (-11.3±1.4μM vs. -7.1±5.5μM) for MA compared to YA.Conclusions: During a high volume, resistance exercise protocol, middle-aged adults experienced reduced muscle oxygen saturation levels compared to younger, recreationally active adults. These results suggest that age-related changes in muscle oxygenation appear to occur in middle-aged adults, regardless of recreational exercise training experience.

中文翻译：

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大量抵抗运动期间年轻和中年娱乐活动男性之间的肌肉氧合作用差异

目的：本研究的目的是比较年轻成年人（YA）和中年成年人（MA）男性在大容量，等运动阻力锻炼协议（HVP）期间股外侧肌的肌肉氧合作用。方法：二十名接受过休闲训练的男性被分为YA（21.8±2.0 y，90.7±11.6 kg，179±4.7 cm）或MA（47.0±4.4 y，96.1±21.6 kg，177±7.7 cm）组。HVP包括八组，每组10次重复，分别为60°·sec-1的单侧等速同心膝盖伸展和偏心膝盖屈曲。在运动过程中，使用近红外光谱法（NIRS）测量了组织血红蛋白饱和度指数（TSI），组织氧合血红蛋白浓度（O2Hb），脱氧血红蛋白（HHb）和肌肉氧化指数（O2Hb-HHb）的变化。使用方差的双向混合因子分析来分析数据。结果：运动前，YA的TSI明显高于MA（73.2±3.4％vs. 68.6±4.8％，p = 0.024）。在两组中，相对于其余时间段，两组的O2Hb和O2Hb-HHb均显着降低，而HHb升高（p <0.05）。然而，八组HVP的平均变化显示HHb水平明显较高（p = 0.036）（7.8±0.5μMvs. 5.3±3.1μM），而O2Hb-HHb水平较低（p = 0.029）（与YA相比，MA的-11.3±1.4μM相对于-7.1±5.5μM）结论：在高容量抗阻力锻炼方案中，中年成年人的肌肉氧饱和度水平低于年轻，具有娱乐活动能力的成年人。这些结果表明，无论休闲运动训练的经历如何，与年龄相关的肌肉氧合作用似乎都发生在中年成年人中。