Metabolic pathways participating in adenosine triphosphate (ATP) synthesis play an important role in lymphocytes normal development and functioning. Energetic metabolism has an influence on differentiation, proliferation and fate of lymphocytes. The aim of the study is to define ATP availability of peripheral blood lymphocytes and the level of mitochondrial metabolism regulator sirtuin 3 (SIRT3) in healthy northerners. Methods. 106 volunteers, habitants of Arkhangelsk (76 women and 30 men, 21-63 years old) were surveyed. Amounts of immunocompetent cells with receptors (CD3, CD4, CD8, CD10, CD16, CD71, CD23, CD25, HLA DR, CD95) were determined by indirect immunoperoxidase method, ATP concentration in lymphocytes by bioluminescent method, and additionally SIRT3 (23 volunteers) by enzyme immunoassay. Two groups were selected using "k-means" cluster analysis which differed statistically significantly in all studied parameters. Results. It was found that 78 % of the surveyed participants were in the group where ATP concentration was 0,95 (0,487) mcmol/106 cells, in other group with 22 % participants it was 3,71 (1,319) mcmol/106 cells, р < 0,0001. Difference in relative amounts of particular cell phenotypes was revealed in the mentioned groups: in the group with lower ATP levels the higher was percentage of CD95+, CD23+ and HLA DR cells. In the group with higher ATP levels the higher was percentage of CD3+, CD4+, CD8+, CD71+ cells and shift of CD10/CD95 and CD4/CD23 ratios was also detected in favor of CD10+ и CD4+ cells with high levels of metabolic activity. Difference of ATP levels was omni-directional with SIRT3, regulator of mitochondrial metabolism, level with concentrations 0,15 (0,039) and 0,39 (0,198) pg/106 cells, р = 0,0097. Conclusion. Intersectionality of ATP availability and reactivity of T cells was identified. It appears to be promising to determine the level of ATP for evaluation of lymphocytes functioning.

中文翻译：

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北欧俄罗斯居民外周血淋巴细胞三磷酸腺苷的可用性

参与三磷酸腺苷 (ATP) 合成的代谢途径在淋巴细胞的正常发育和功能中起着重要作用。能量代谢对淋巴细胞的分化、增殖和命运有影响。该研究的目的是确定健康北方人外周血淋巴细胞的 ATP 可用性和线粒体代谢调节剂 Sirtuin 3 (SIRT3) 的水平。方法。106 名志愿者，阿尔汉格尔斯克居民（76 名女性和 30 名男性，21-63 岁）接受了调查。通过间接免疫过氧化物酶法测定具有受体（CD3、CD4、CD8、CD10、CD16、CD71、CD23、CD25、HLA DR、CD95）的免疫活性细胞的数量，通过生物发光法测定淋巴细胞中的 ATP 浓度，另外还有 SIRT3（23 名志愿者）通过酶免疫分析。使用“k-means”选择两组 聚类分析在所有研究参数中具有统计学显着差异。结果。发现 78 % 的被调查参与者处于 ATP 浓度为 0,95 (0,487) mcmol/106 个细胞的组中，在其他组中，22% 的参与者为 3,71 (1,319) mcmol/106 个细胞，р < 0,0001。在上述组中揭示了特定细胞表型的相对数量的差异：在具有较低 ATP 水平的组中，CD95+、CD23+ 和 HLA DR 细胞的百分比较高。在具有较高 ATP 水平的组中，CD3+、CD4+、CD8+、CD71+ 细胞的百分比较高，并且还检测到 CD10/CD95 和 CD4/CD23 比率的变化，有利于具有高水平代谢活性的 CD10+ 和 CD4+ 细胞。ATP 水平的差异与线粒体代谢调节剂 SIRT3 是全方位的，浓度为 0,15 (0,039) 和 0,39 (0,198) pg/106 个细胞的水平，р = 0,0097。结论。确定了 ATP 可用性和 T 细胞反应性的交叉性。确定 ATP 水平以评估淋巴细胞功能似乎是有希望的。