The emerging intestinal pathogen Campylobacter concisus has been associated with prolonged diarrhoea and classic inflammatory bowel diseases (IBD) and was recently also linked with microscopic colitis (MC). Previous reports have observed a high genetic diversity within isolates from diarrhoeic and IBD patients and from healthy controls (HC), and division of isolates into two major genomospecies (GS1 and GS2). The aim of this study was to describe genetic diversity in 80 recently cultivated MC biopsy and faecal isolates of C. concisus by multi-locus sequence typing (MLST); and to compare the phylogenetic relatedness to 102 isolates from diarrhoeic and IBD patients and HCs by k-mer-based distance estimation. MLST revealed high genetic diversity in MC isolates with 72 novel sequence types. K-mer divided MC isolates into two distinct clusters (cluster 1 n = 21, cluster 2 n = 49), with a significantly higher prevalence of cluster 2 isolates in biopsies than in faeces, p = 0.009. K-mer divided the 182 isolates into two major phylogenetic clusters: cluster 1 (GS1 isolates) and cluster 2 (GS2 isolates), which further differentiated into three subgroups. Cluster 1 and the three cluster 2 subgroups were each distinctive in mean genome size and GC count. Isolates from all disease phenotypes were present in cluster 1 and cluster 2 subgroup 2 and 3, whereas cluster 2 subgroup 1 only contained isolates restricted to patients with ulcerative colitis (n = 10) and HC (n = 4).

中文翻译：

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显微镜下结肠炎患者结膜弯曲杆菌中高遗传多样性

新兴的肠道病原体弯曲杆菌弯曲杆菌与长时间的腹泻和经典的炎症性肠病（IBD）有关，最近还与微观结肠炎（MC）相关。以前的报道已经观察到腹泻和IBD患者以及健康对照（HC）的分离株中存在高度的遗传多样性，并且将分离株分为两个主要基因组（GS1和GS2）。这项研究的目的是通过多基因座序列分型（MLST）描述最近培养的80种MC活检组织和浓缩锥状杆菌粪便分离株的遗传多样性。并通过基于k-mer的距离估算来比较腹泻和IBD患者与HCs的102种分离株的系统发育相关性。MLST揭示了MC分离物中具有72种新序列类型的高度遗传多样性。K-mer将MC分离株分为两个不同的簇（簇1 n = 21，簇2 n = 49），活检中簇2分离株的患病率明显高于粪便，p = 0.009。K-mer将182个分离株分为两个主要的系统发育簇：第1个簇（GS1分离株）和第2个簇（GS2分离株），它们进一步分为三个亚组。聚类1和三个聚类2亚组在平均基因组大小和GC计数上各不相同。来自所有疾病表型的分离株分别存在于第1组，第2组第2和第3组，而第2组第1组仅包含仅限于溃疡性结肠炎（n = 10）和HC（n = 4）患者的分离株。K-mer将182个分离株分为两个主要的系统发育簇：第1个簇（GS1分离株）和第2个簇（GS2分离株），它们进一步分为三个亚组。聚类1和三个聚类2亚组在平均基因组大小和GC计数上各不相同。来自所有疾病表型的分离株分别存在于第1组，第2组第2和第3组，而第2组第1组仅包含仅限于溃疡性结肠炎（n = 10）和HC（n = 4）患者的分离株。K-mer将182个分离株分为两个主要的系统发育簇：第1个簇（GS1分离株）和第2个簇（GS2分离株），它们进一步分为三个亚组。聚类1和三个聚类2亚组在平均基因组大小和GC计数上各不相同。来自所有疾病表型的分离株分别存在于第1组，第2组第2和第3组，而第2组第1组仅包含仅限于溃疡性结肠炎（n = 10）和HC（n = 4）患者的分离株。