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An SPR imaging immunosensor for leptin determination in blood plasma
Analytical Methods ( IF 2.7 ) Pub Date : 2021-1-5 , DOI: 10.1039/d0ay02047h Anna Sankiewicz 1, 2, 3, 4, 5 , Adam Hermanowicz 4, 5, 6, 7 , Artur Grycz 1, 2, 3, 4, 5 , Zenon Łukaszewski 5, 8, 9, 10 , Ewa Gorodkiewicz 1, 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2021-1-5 , DOI: 10.1039/d0ay02047h Anna Sankiewicz 1, 2, 3, 4, 5 , Adam Hermanowicz 4, 5, 6, 7 , Artur Grycz 1, 2, 3, 4, 5 , Zenon Łukaszewski 5, 8, 9, 10 , Ewa Gorodkiewicz 1, 2, 3, 4, 5
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Leptin is a hormone that has a fundamental role in the regulation of feeding and energy balance. A developed specific SPRi immunosensor for leptin may be a new tool for leptin determination in blood plasma. The immunosensor consists of rabbit anti-leptin antibody immobilized on a gold chip via cysteamine linker, using the EDC/NHS protocol. Non-fluidic array SPRi is used for analytical signal formation. Under optimized conditions, the linear response range of the immunosensor covers concentrations from 0.23 to 5 ng mL−1. The LOD of the immunosensor is 0.07 ng mL−1, and the LOQ is 0.23 ng mL−1. The precision of measurement depends on leptin concentration, and is between 9.1% and 2.2%. Recoveries of the leptin spike are between 97% and 110%. The immunosensor and related analytical method were validated by parallel determination of leptin in series of plasma from children suffering from malnutrition and a control group, using Enzyme-Linked Immunosorbent Assay (ELISA) and SPRi. Pearson's correlation coefficient was equal to 0.991. The developed immunosensor and related method are more direct, faster and much simpler than ELISA.
中文翻译：
用于血浆中瘦素测定的SPR成像免疫传感器
瘦素是一种激素，在调节饲喂和能量平衡方面具有基本作用。针对瘦素的已开发的特异性SPRi免疫传感器可能是测定血浆中瘦素的新工具。免疫传感器由兔抗瘦素抗体组成，该抗体使用EDC / NHS方案通过半胱胺接头固定在金芯片上。非流体阵列SPRi用于分析信号形成。在最佳条件下，免疫传感器的线性响应范围涵盖0.23至5 ng mL -1的浓度。免疫传感器的LOD为0.07 ng mL -1，LOQ为0.23 ng mL -1。测量精度取决于瘦素浓度，介于9.1％和2.2％之间。瘦素峰值的回收率在97％至110％之间。通过酶联免疫吸附测定（ELISA）和SPRi，通过平行测定营养不良儿童和对照组血浆中的瘦素水平，验证了免疫传感器和相关分析方法的有效性。皮尔逊的相关系数等于0.991。所开发的免疫传感器和相关方法比ELISA更直接，更快，更简单。
更新日期：2021-01-13
中文翻译：
用于血浆中瘦素测定的SPR成像免疫传感器
瘦素是一种激素，在调节饲喂和能量平衡方面具有基本作用。针对瘦素的已开发的特异性SPRi免疫传感器可能是测定血浆中瘦素的新工具。免疫传感器由兔抗瘦素抗体组成，该抗体使用EDC / NHS方案通过半胱胺接头固定在金芯片上。非流体阵列SPRi用于分析信号形成。在最佳条件下，免疫传感器的线性响应范围涵盖0.23至5 ng mL -1的浓度。免疫传感器的LOD为0.07 ng mL -1，LOQ为0.23 ng mL -1。测量精度取决于瘦素浓度，介于9.1％和2.2％之间。瘦素峰值的回收率在97％至110％之间。通过酶联免疫吸附测定（ELISA）和SPRi，通过平行测定营养不良儿童和对照组血浆中的瘦素水平，验证了免疫传感器和相关分析方法的有效性。皮尔逊的相关系数等于0.991。所开发的免疫传感器和相关方法比ELISA更直接，更快，更简单。
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