Orthosiphon stamineus Benth. (Lamiaceae) is commonly used for the treatment of kidney diseases, but its role in intestinal barrier function remains unknown. The present study investigated the protective effects of O. stamineus extract (OE) against oxidative stress-induced injury to the small intestinal epithelium and the possible mechanism. High-performance liquid chromatography fingerprinting was used to analyze OE. Oxidative stress was induced by hydrogen peroxide (1 mM for 1 hour) in an IPEC-J2 cell monolayer model and a high-fat diet in C57BL/6 mice (8 weeks). The malondialdehyde (MDA) content was tested in both models. To evaluate permeability, transepithelial electrical resistance (TEER) was tested in a cell model. Serum diamine oxidase (DAO) and endotoxin contents were determined in a mouse model, and histological sections were analyzed. The messenger ribonucleic acid expression of tight junction proteins was measured by quantitative real-time polymerase chain reaction. Pretreatment with OE (50 µg/mL) increased the IPEC-J2 cell monolayer TEER (12.4%) and decreased MDA (from 6.1 to 4.7 mmol/mg prot). Oral administration of OE (100 mg/kg) decreased serum DAO (34.2%), endotoxin (13.4%), and MDA (from 21.3 to 11.0 mmol/mL) in mice. OE upregulated ZO-1 (42.8% in the cell model and 125.0% in mice) and occluding (127.0% in the cell model and 120.3% in mice) gene expression. These results confirmed the protective effect of OE on the intestinal barrier, which was associated with the antioxidant effect of OE; thus, OE is suitable for the prevention and treatment of intestinal barrier injury.

Orthosiphon stamineus Benth。（唇形科）通常用于治疗肾脏疾病，但其在肠屏障功能中的作用仍然未知。本研究调查了稻瘟病菌的保护作用提取物（OE）抗氧化应激诱导的小肠上皮损伤及其可能的机制。高效液相色谱指纹图谱用于分析OE。在IPEC-J2细胞单层模型和C57BL / 6小鼠的高脂饮食（8周）中，过氧化氢（1 mM，持续1小时）诱导了氧化应激。在两个模型中均测试了丙二醛（MDA）含量。为了评估渗透性，在细胞模型中测试了跨上皮电阻（TEER）。测定小鼠模型中的血清二胺氧化酶（DAO）和内毒素含量，并分析组织学切片。通过定量实时聚合酶链反应测量紧密连接蛋白的信使核糖核酸表达。用OE（50 µg / mL）预处理可增加IPEC-J2细胞单层TEER（12。4％）和MDA降低（从6.1降至4.7 mmol / mg脯氨酸）。口服OE（100 mg / kg）会降低小鼠的血清DAO（34.2％），内毒素（13.4％）和MDA（从21.3至11.0 mmol / mL）。OE上调ZO-1（在细胞模型中为42.8％，在小鼠中为125.0％）并阻塞（在细胞模型中为127.0％，在小鼠中为120.3％）基因表达。这些结果证实了OE对肠屏障的保护作用，这与OE的抗氧化作用有关。因此，OE适合于肠屏障损伤的预防和治疗。这些结果证实了OE对肠屏障的保护作用，这与OE的抗氧化作用有关。因此，OE适合于肠屏障损伤的预防和治疗。这些结果证实了OE对肠屏障的保护作用，这与OE的抗氧化作用有关。因此，OE适合于肠屏障损伤的预防和治疗。