Escherichia coli O157:H7 (E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 10⁵ CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

大肠杆菌O157:H7（E.coli O157:H7）是一种危险的食源性病原体，主要存在于牛肉、牛奶、水果及其制品中，对人体健康造成危害甚至死亡。因此，食品中大肠杆菌O157:H7的检测尤为重要。在本文中，我们报告了一种基于聚集诱导发射 (AIE) 材料标记抗原的侧流免疫测定条 (LFIS) 作为荧光探针，用于快速检测大肠杆菌O157:H7。该试纸条的检测灵敏度为10 ⁵ CFU/mL，比胶体金试纸条高10倍。该方法具有良好的特异性和稳定性，可用于检测约250 CFU的大肠杆菌O157:H7 成功加入 25 g 或 25 mL 牛肉、果冻和牛奶中。AIE-LFIS 在监测食品病原体以进行快速检测方面可能很有价值。