Optical resolution photoacoustic microscopy (ORPAM) has demonstrated both high resolution and rich contrast imaging of optical chromophores in biologic tissues. To date, sensitivity remains a major challenge for ORPAM, which limits the capability of resolving biologic microvascular networks. In this study, we propose and evaluate a new ORPAM modality termed as optical resolution photoacoustic computed microscopy (ORPACM), through the combination of a two-dimensional laser-scanning system with a medical ultrasonographic platform. Apart from conventional ORPAMs, we record multiple photoacoustic (PA) signals using a 128-element ultrasonic transducer array for each pulse excitation. Then, we apply a reconstruction algorithm to recover one depth-resolved PA signal referred to as an A-line, which reveals more detailed information compared with conventional single-element transducer-based ORPAMs. In addition, we carried out both in vitro and in vivo experiments as well as quantitative analyses to show the advanced features of ORPACM.

中文翻译：

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光学分辨率光声计算机显微镜

光学分辨率光声显微镜 (ORPAM) 已经证明了生物组织中光学发色团的高分辨率和丰富的对比度成像。迄今为止，灵敏度仍然是 ORPAM 的主要挑战，这限制了解析生物微血管网络的能力。在这项研究中，我们通过将二维激光扫描系统与医学超声平台相结合，提出并评估了一种称为光学分辨率光声计算机显微镜 (ORPACM) 的新 ORPAM 模式。除了传统的 ORPAM，我们还使用 128 个元件的超声换能器阵列为每个脉冲激发记录多个光声 (PA) 信号。然后，我们应用重建算法来恢复一个深度解析的 PA 信号，称为 A 线，与传统的基于单晶换能器的 ORPAM 相比，它揭示了更详细的信息。此外，我们还进行了体外和体内实验以及定量分析以展示 ORPACM 的高级功能。