Schlegelella thermodepolymerans is a moderately thermophilic bacterium capable of producing polyhydroxyalkanoates—biodegradable polymers representing an alternative to conventional plastics. Here, we present the first complete genome of the type strain S. thermodepolymerans DSM 15344 that was assembled by hybrid approach using both long (Oxford Nanopore) and short (Illumina) reads. The genome consists of a single 3,858,501-bp-long circular chromosome with GC content of 70.3%. Genome annotation identified 3,650 genes in total, whereas 3,598 open reading frames belonged to protein-coding genes. Functional annotation of the genome and division of genes into clusters of orthologous groups revealed a relatively high number of 1,013 genes with unknown function or unknown clusters of orthologous groups, which reflects the fact that only a little is known about thermophilic polyhydroxyalkanoates-producing bacteria on a genome level. On the other hand, 270 genes involved in energy conversion and production were detected. This group covers genes involved in catabolic processes, which suggests capability of S. thermodepolymerans DSM 15344 to utilize and biotechnologically convert various substrates such as lignocellulose-based saccharides, glycerol, or lipids. Based on the knowledge of its genome, it can be stated that S. thermodepolymerans DSM 15344 is a very interesting, metabolically versatile bacterium with great biotechnological potential.

中文翻译：

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嗜热聚羟基链烷酸酯产生细菌的第一个完整基因组 Schlegelella thermodepolymerans DSM 15344

Schlegelella thermodepolymerans 是一种中等嗜热细菌，能够产生聚羟基链烷酸酯——可生物降解的聚合物，代表了传统塑料的替代品。在这里，我们展示了 S. thermodepolymerans DSM 15344 型菌株的第一个完整基因组，该基因组通过混合方法使用长 (Oxford Nanopore) 和短 (Illumina) 读数进行组装。该基因组由一条 3,858,501 bp 长的环状染色体组成，GC 含量为 70.3%。基因组注释总共鉴定了 3,650 个基因，而 3,598 个开放阅读框属于蛋白质编码基因。基因组的功能注释和将基因划分为直系同源群簇揭示了数量相对较多的 1,013 个具有未知功能或未知直系同源群簇的基因，这反映了一个事实，即在基因组水平上对嗜热聚羟基链烷酸酯产生细菌知之甚少。另一方面，检测到270个参与能量转换和生产的基因。该组涵盖了参与分解代谢过程的基因，这表明 S. thermodepolymerans DSM 15344 能够利用和生物技术转化各种底物，例如基于木质纤维素的糖类、甘油或脂质。基于对其基因组的了解，可以说 S. thermodepolymerans DSM 15344 是一种非常有趣的、代谢多才多艺的细菌，具有巨大的生物技术潜力。该组涵盖了参与分解代谢过程的基因，这表明 S. thermodepolymerans DSM 15344 能够利用和生物技术转化各种底物，例如基于木质纤维素的糖类、甘油或脂质。基于对其基因组的了解，可以说 S. thermodepolymerans DSM 15344 是一种非常有趣的、代谢多才多艺的细菌，具有巨大的生物技术潜力。该组涵盖了参与分解代谢过程的基因，这表明 S. thermodepolymerans DSM 15344 能够利用和生物技术转化各种底物，例如基于木质纤维素的糖类、甘油或脂质。基于对其基因组的了解，可以说 S. thermodepolymerans DSM 15344 是一种非常有趣的、代谢多才多艺的细菌，具有巨大的生物技术潜力。