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Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration
Frontiers in Bioengineering and Biotechnology ( IF 4.3 ) Pub Date : 2021-01-13 , DOI: 10.3389/fbioe.2020.595096
Jiahui Fu , Jianfeng Chen , Wenjun Li , Xiaomin Yang , Jingyan Yang , Huixin Quan , Haitao Huang , Gang Chen

Native dental pulp extracellular matrix (DPEM) has proven to be an effective biomaterial for dental pulp regeneration. However, as a significant extracellular matrix glycoprotein, partial laminins were lost during the decellularization process, which were essential for odontoblast differentiation. Thereby, this study investigated the feasibility of LN supplementation to improve the surface of DPEM for odontoblast layer regeneration. The influences of laminin on cell adhesion and odontogenic differentiation were evaluated in vitro. Then, we fabricated laminin-modified DPEM based on the physical coating strategy and observed the location and persistency of laminin coating by immunofluorescent staining. Finally, laminin-modified DPEM combined with treated dentin matrix (TDM) was transplanted in orthotopic jaw bone of beagles (n = 3) to assess the effect of LNs on dental pulp tissue regeneration. The in vitro results showed that laminins could improve the adhesion of dental pulp stem cells (DPSCs) and promoted DPSCs toward odontogenic differentiation. Continuous odontoblastic layer-like structure was observed in laminin-modified DPEM group, expressing the markers for odontoblastogenesis, dentine matrix protein-1 (DMP-1) and dentin sialophosphoprotein (DSPP). Overall, these studies demonstrate that the supplementation of laminins to DPEM contributes to the odontogenic differentiation of cells and to the formation of odontoblast layer in dental pulp regeneration.

中文翻译:

用于牙髓再生的层粘连蛋白修饰的牙髓细胞外基质

天然牙髓细胞外基质 (DPEM) 已被证明是一种有效的牙髓再生生物材料。然而,作为一种重要的细胞外基质糖蛋白,部分层粘连蛋白在脱细胞过程中丢失,这对成牙本质细胞分化至关重要。因此,本研究调查了补充 LN 以改善 DPEM 表面以促进成牙本质细胞层再生的可行性。体外评价层粘连蛋白对细胞粘附和牙源性分化的影响。然后,我们基于物理涂层策略制备了层粘连蛋白修饰的 DPEM,并通过免疫荧光染色观察了层粘连蛋白涂层的位置和持久性。最后,将层粘连蛋白修饰的 DPEM 结合处理过的牙本质基质 (TDM) 移植到比格犬 (n = 3) 的原位颌骨中,以评估 LNs 对牙髓组织再生的影响。体外实验结果表明,层粘连蛋白可以提高牙髓干细胞(DPSCs)的粘附力,促进DPSCs向牙源性分化。在层粘连蛋白修饰的DPEM组中观察到连续的成牙本质层状结构,表达成牙本质细胞发生的标志物牙本质基质蛋白1(DMP-1)和牙本质唾液酸磷蛋白(DSPP)。总的来说,这些研究表明,向 DPEM 补充层粘连蛋白有助于细胞的牙源性分化和牙髓再生中成牙本质细胞层的形成。体外实验结果表明,层粘连蛋白可以提高牙髓干细胞(DPSCs)的粘附力,促进DPSCs向牙源性分化。在层粘连蛋白修饰的DPEM组中观察到连续的成牙本质层状结构,表达成牙本质细胞发生的标志物牙本质基质蛋白1(DMP-1)和牙本质唾液酸磷蛋白(DSPP)。总的来说,这些研究表明,向 DPEM 补充层粘连蛋白有助于细胞的牙源性分化和牙髓再生中成牙本质细胞层的形成。体外实验结果表明,层粘连蛋白可以提高牙髓干细胞(DPSCs)的粘附力,促进DPSCs向牙源性分化。在层粘连蛋白修饰的DPEM组中观察到连续的成牙本质层状结构,表达成牙本质细胞发生的标志物牙本质基质蛋白1(DMP-1)和牙本质唾液酸磷蛋白(DSPP)。总的来说,这些研究表明,向 DPEM 补充层粘连蛋白有助于细胞的牙源性分化和牙髓再生中成牙本质细胞层的形成。牙本质基质蛋白-1 (DMP-1) 和牙本质唾液酸磷蛋白 (DSPP)。总的来说,这些研究表明,向 DPEM 补充层粘连蛋白有助于细胞的牙源性分化和牙髓再生中成牙本质细胞层的形成。牙本质基质蛋白-1 (DMP-1) 和牙本质唾液酸磷蛋白 (DSPP)。总的来说,这些研究表明,向 DPEM 补充层粘连蛋白有助于细胞的牙源性分化和牙髓再生中成牙本质细胞层的形成。
更新日期:2021-01-13
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