Previous studies have suggested that 1,9-Pyrazoloanthrone, known as SP600125, can induce cell polyploidization. However, what is the phase of cell cycle arrest caused by SP600125 and the underlying regulation is still an interesting issue to be further addressed. Research in this article shows that SP600125 can block cell cycle progression at the prometaphase of mitosis and cause endomitosis. It is suggested that enhancement of the p53 signaling pathway and weakening of the spindle assembly checkpoint are associated with the SP600125-induced cell cycle arrest. Using preliminary SP600125 treatment, the samples of the cultured fish cells and the fish tissues display a great number of chromosome splitting phases. Summarily, SP600125 can provide a new protocol of chromosomes preparation for karyotype analysis owing to its interference with prometaphase of mitosis.

中文翻译：

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ATP竞争性抑制剂SP600125通过增强鱼类内丝体制备制备染色体的新方法

先前的研究表明，称为 SP600125 的 1,9-吡唑并蒽酮可以诱导细胞多倍化。然而，SP600125引起的细胞周期停滞的阶段是什么以及潜在的调控仍然是一个有待进一步解决的有趣问题。这篇文章的研究表明，SP600125 可以在有丝分裂前期阻断细胞周期进程，并引起内丝分裂。这表明 p53 信号通路的增强和纺锤体组装检查点的减弱与 SP600125 诱导的细胞周期停滞有关。使用 SP600125 初步处理，培养的鱼细胞和鱼组织样本显示出大量的染色体分裂阶段。概括地说，