Lactobacillus strains dominate the vaginal habitat and they are associated with a lower risk of genital infections. In addition, they contribute to the conservation of the vaginal microbiota by producing postbiotic agents. Previous studies have shown that their predominance involves antimicrobial activity against urogenital pathologies. In this context, probiotics may improve treatment outcomes. The aim of this study was to evaluate the probiotic properties of lactobacilli strains of vaginal origin using a multidisciplinary approach. For this purpose, safety criteria, ability to resist at low pH and bile salts, antimicrobial activity, ability to produce biofilm, capacity to produce hydrogen peroxide and more importantly, auto-aggregation, co-aggregation (with Candida spp.) and adhesion to human cells were evaluated. The strains belonged to the species of L. crispatus, L. gasseri, L. rhamnosus and L. delbruckii. Among these, a strain of L. rhamnosus named AD3 showed the best probiotic properties. As probiotics are already in use in many clinical practice and there are no major safety concerns, L. rhamnosus AD3 showed promise in becoming a prevention and complementary treatment option for urogenital diseases. Indeed, these results suggest that strain L. rhamnosus AD3 is non-pathogenic and likely to be safe for human consumption. This study revealed the great amensalistic properties of a new L. rhamnosus strain which can aim to be used as probiotic in pharmaceutical applications.

中文翻译：

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鼠李糖乳杆菌AD3可作为益生菌产品的替代品

乳酸杆菌菌株在阴道生境中占主导地位，它们与生殖器感染的风险较低有关。另外，它们通过产生后生因子来促进阴道微生物群的保存。先前的研究表明，它们的优势涉及针对泌尿生殖道疾病的抗菌活性。在这种情况下，益生菌可以改善治疗效果。这项研究的目的是使用多学科方法评估阴道来源的乳酸杆菌菌株的益生菌特性。为此，需要考虑安全性标准，在低pH和胆汁盐中的抵抗能力，抗菌活性，产生生物膜的能力，产生过氧化氢的能力，更重要的是，自动聚集，共同聚集（与假丝酵母spp。）和对人体细胞的粘附性进行了评估。菌株属于的种类卷曲乳杆菌，加氏乳杆菌，鼠李糖乳杆菌和L.德氏。其中，命名为AD3的鼠李糖乳杆菌菌株表现出最佳的益生菌特性。由于益生菌已经在许多临床实践中使用，并且没有重大的安全隐患，鼠李糖乳杆菌AD3在成为泌尿生殖道疾病的预防和补充治疗选择方面显示出了希望。实际上，这些结果表明，鼠李糖乳杆菌AD3菌株是非致病性的，可能对人类食用是安全的。这项研究揭示了一种新的鼠李糖鼠的绝育特性。 可以在药物应用中用作益生菌的菌株。