Abstract

Hypericum perforatum is a useful plant because of its biological features, such as bactericidal, virucidal, and fungicidal effects. Elimination of periodontal pathogens, including Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis, from the oral environment, is important for the stability of periodontal health. Therefore, we aimed to determine the antimicrobial activity of the Hypericum perforatum L. essential oils over these pathogens using cell cultures. The standard and clinical bacterial strains were used in this study. The essential oils extracted from Hypericum perforatum L. flowers were analyzed with gaschromatography/mass spectrometry. The antimicrobial activities of the essential oils along with 0.2 % chlorhexidine, 10 % povidone-iodine, and combinations of essential oils and 0.2 % chlorhexidine, and essential oils and 10 % povidone-iodine were evaluated by measuring the minimum inhibitory concentrations. Essential oils’ minimum inhibitory concentration value for clinical A. actinomycetemcomitans strains was significantly lower than that of the povidone-iodine solution (P<0.05). However, despite the low minimum inhibitory concentration value of essential oils, when compared to 0.2 % chlorhexidine, there was no statistically significant difference between the agents (P=0.113). For clinical strains of Porphyromonas gingivalis, the essential oils showed a lower minimum inhibitory concentration value compared to chlorhexidine and povidone-iodine. The essential oils combined with chlorhexidine or povidone-iodine showed significantly lower minimum inhibitory concentration value for both strains of Aggregatibacter actinomycetemcomitans (P<0.05). With the lower minimum inhibitory concentration values, the essential oils showed strong antimicrobial activity. Moreover, when combined with chlorhexidine or the povidone-iodine, the antimicrobial effect of essential oils on Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis strains increased synergistically.

摘要

贯叶连翘由于其生物学特性，例如杀菌，杀病毒和杀真菌作用，是一种有用的植物。从口腔环境中消除牙周病原体，包括放线菌，放线菌和牙龈卟啉单胞菌，对于稳定牙周健康很重要。因此，我们旨在通过细胞培养来确定贯叶连翘精油对这些病原体的抗菌活性。在这项研究中使用了标准菌株和临床细菌菌株。从贯叶连翘提取的香精油用气相色谱/质谱法分析L.花。通过测量最小抑菌浓度，评估了香精油与0.2％洗必太，10％聚维酮碘，以及精油和0.2％氯己定的组合以及香精油和10％聚维酮碘的抗菌活性。精油对临床放线放线杆菌菌株的最低抑菌浓度值明显低于聚维酮碘溶液（P <0.05）。然而，尽管精油的最低抑菌浓度值较低，但与0.2％的洗必泰相比，两种药剂之间没有统计学上的显着差异（P = 0.113）。对于临床菌株与氯己定和聚维酮-碘相比，齿龈卟啉单胞菌的最低抑菌浓度值更低。香精油与洗必泰或聚维酮碘合用对两种菌株放线杆菌的最低抑菌浓度值均显着降低（P <0.05）。随着最低抑菌浓度值的降低，香精油显示出强大的抗菌活性。此外，当与洗必太或聚维酮碘合用时，香精油对集合放线杆菌和牙龈卟啉单胞菌菌株的抗菌作用协同增加。