Abstract

Artemisia fragrans is an aromatic and perennial herb with green silver leaves and yellow flowers, widely distributed in Iran, Azerbaijan, Russia and Turkey. The plant and its essential oil (EO) are used in traditional medicine and have economic value. In this study, the composition, antibacterial and antioxidant activities of 16 A. fragrans essential oil samples collected from different habitats in Iran were analyzed. A total of 85 compounds were detected using GC-MS analysis, the major constituents being camphor (9.91-34.44 %), α-thujone (19.22-42.63 %) and 1,8-cineole (12.57-31.87 %). Davanone D, α-cadinol, verbenene, ortho-oci-men were detected in the analysis as the new components for this species and are first reported by us. Anti-bacterial activity and minimum inhibitory and bactericidal concentrations of EOs were tested on Escherichia coli, Klebsiella pneumonia and Proteus vulgaris (Gram-negative strains) Staphylococcus aureus, Staphylococ-cus epidermidis and Bacillus subtilis (Gram-positive strains). The EOs showed better inhibitory effects against Gram-negative strains compared to Gram-positive ones. Among the EOs, E7 (IC₅₀ = 9.1 μg/mL) population showed the highest radical scavenging capacity, which was equal to the positive control (butylated hyroxytoluene). The quantitative and qualitative variability of the studied EOs and their antibacterial and antioxidant activities may be recommended for pharmacological and cosmetic industries.

摘要

黄蒿是一种多年生芳香植物，带有绿色的银叶和黄色的花，广泛分布于伊朗，阿塞拜疆，俄罗斯和土耳其。该植物及其精油（EO）用于传统医学，具有经济价值。在这项研究中，分析了从伊朗不同生境收集的16种苦艾茶精油样品的成分，抗菌和抗氧化活性。使用GC-MS分析检测到总共85种化合物，主要成分为樟脑（9.91-34.44％），α-丁酮（19.22-42.63％）和1,8-桉树脑（12.57-31.87％）。在分析中检测到达万烷D，α-cadinol，黄苯烯，邻位缔合菌是该物种的新成分，并由我们首先报道。在以下条件下测试了EO的抗菌活性以及最低抑菌和杀菌浓度大肠杆菌，肺炎克雷伯菌和寻常变形杆菌（革兰氏阴性菌株）金黄色葡萄球菌，表皮葡萄球菌和枯草芽孢杆菌（革兰氏阳性菌株）。与革兰氏阳性菌株相比，EOs对革兰氏阴性菌株表现出更好的抑制作用。在EO中，E7（IC ₅₀ = 9.1μg/ mL）群体显示出最高的自由基清除能力，与阳性对照（丁基化羟基甲苯）相当。研究的EOs的数量和质量变异性及其抗菌和抗氧化活性可能被推荐用于药理和化妆品行业。