Abstract:

Ruta chalepensis L. (Rutaceae) is an aromatic herb used in traditional medicine in Jordan and many countries. The main aims of this study are to isolate and identify the components of the essential oil from the aerial parts of R. chalepensis. Determination of the total phenolic and flavonoid contents, in addition to the in vitro assessment of antioxidant capacity as well as the cytotoxic effect of the essential oil extract on human MCF-7, T47D and Caco-2 cancer cell lines. The oil extract was analyzed using gas chromatography-mass spec- trometry (GC-MS). The major components of the essential oil were 2-cyclohexen-1-one,3-[(2,3,4,9 tetrahydro 1H pyrido[3,4 b]indole-1-yl) methyl]- (45.97 %) and 2-Nonanone (19.45 %). The total phenolic was 5.00 ± 0.005 mg gallic acid equivalent/ g of oil extract, while the total flavonoid content was 34.09 ± 0.140 mg quercetin equivalent/ g oil. The antioxidant properties were screened using DPPH radical scavenging assay (IC₅₀ was 35.27 μg/ml) and the reducing power ability (EC₅₀ was 20110 μg/ml). The cytotoxic activity was assessed using the MTT assay. The cytotoxicity of oil extract in all tested cell lines was in a dose-dependent manner. The calculated IC₅₀ values of the oil extract against MCF-7, T47D and Caco-2 (79.32±0.948 μg/ml, 97.23±0.57 μg/ml, 107.72±1.85 μg/ml), respectively. Furthermore, there was no significant inhibitory effect of the oil extract on the normal human fibro-blast cell line (MRC-5). This study demonstrated that R. chalepensis essential oil possesses antioxidant activity and could be considered as a potential source of the anticancer compound.

摘要：

Ruta chalepensis L.（芸香科）是约旦和许多国家的传统医学中使用的芳香草药。这项研究的主要目的是从沙棘的地上部分分离和鉴定精油的成分。除了体外测定总酚和类黄酮含量评估抗氧化能力以及精油提取物对人MCF-7，T47D和Caco-2癌细胞系的细胞毒性作用。使用气相色谱-质谱仪（GC-MS）分析油提取物。精油的主要成分是2-环己烯-1-酮，3-[（2,3,4,9四氢1H吡啶基[3,4 b]吲哚-1-基）甲基]-（45.97％）和2-壬基酮（19.45％）。总酚含量为5.00±0.005 mg没食子酸当量/ g油提取物，而总黄酮含量为34.09±0.140 mg槲皮素当量/ g油。使用DPPH自由基清除试验（IC ₅₀为35.27μg/ ml）和还原能力（EC ₅₀）筛选抗氧化剂性能为20110μg/ ml）。使用MTT测定法评估细胞毒性活性。油提取物在所有测试的细胞系中的细胞毒性均呈剂量依赖性。计算出的油提取物针对MCF-7，T47D和Caco-2的IC ₅₀值（分别为79.32±0.948μg/ ml，97.23±0.57μg/ ml，107.72±1.85μg/ ml）。此外，油提取物对正常人成纤维细胞系（MRC-5）没有明显的抑制作用。这项研究表明，沙棘菜精油具有抗氧化活性，可以被认为是抗癌化合物的潜在来源。