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Lactoferrin suppresses LPS-induced expression of HMGB1, microRNA 155, 146, and TLR4/MyD88/NF-кB pathway in RAW264.7 cells
Immunopharmacology and Immunotoxicology ( IF 2.9 ) Pub Date : 2021-01-12
Maryam Nemati, Saeideh Akseh, Maryam Amiri, Hamid Reza Nejabati, Ahmadreza Jodati, Nazila Fathi Maroufi, Yousef Faridvand, Mohammad Nouri

Abstract

Objective

This current study evaluated the underlying mechanisms of LF against the inflammatory microRNAs (miRNAs), HMGB1 expression, and TLR4-MyD88-NF-кB pathway in LPS-activated murine RAW264.7 cells.

Methods

MTT assay was used to assess cell metabolism and the cell culture levels of the cytokines (TNF-α, IL-6) were evaluated by Enzyme-linked immunosorbent assay (ELISA). The expression of miRNAs was quantified by using qPCR and the expression of HMGB1, TLR4, MyD88, and phosphorylated NF-κB (P-p65) were determined with Western blot and qPCR, respectively.

Results

The results indicated that LF downregulates IL-6 and TNF-α expression. LF exhibited the degradation of P-p65 and reduced the production of HMGB1, TLR4, and MyD88 in LPS-induced inflammatory response. Importantly, in parallel with the suppression of cytokines and HMGB1-TLR4-MyD88-NF-кB pathway, LF could induce a decrease in inflammatory selected miRNAs, mmu-mir-155, and mmu-mir-146a expression.

Conclusions

Altogether, these findings provide LF as a prominent anti-inflammatory agent that could modulate HMGB1, mmu-mir-155, mmu-mir-146a, and TLR4/MyD88/NF-кB pathway.



中文翻译:

乳铁蛋白抑制LPS诱导的RAW264.7细胞中HMGB1,microRNA 155、146和TLR4 / MyD88 /NF-кB通路的表达

摘要

目的

这项当前的研究评估了LF抵抗LPS激活的小鼠RAW264.7细胞中炎症性microRNA(miRNA),HMGB1表达和TLR4-MyD88-NF-кB通路的潜在机制。

方法

使用MTT测定法评估细胞代谢,并通过酶联免疫吸附测定法(ELISA)评估细胞因子(TNF-α,IL-6)的细胞培养水平。使用qPCR对miRNA的表达进行定量,分别通过Western blot和qPCR测定HMGB1,TLR4,MyD88和磷酸化的NF-κB(P-p65)的表达。

结果

结果表明LF下调IL-6和TNF-α表达。LF在LPS诱导的炎症反应中表现出P-p65的降解并减少了HMGB1,TLR4和MyD88的产生。重要的是,与抑制细胞因子和HMGB1-TLR4-MyD88-NF-кB途径同时,LF可能会诱导炎性选择的miRNA,mmu-mir -155和mmu-mir -146a表达减少。

结论

总而言之,这些发现提供了LF作为一种重要的抗炎药,可以调节HMGB1,mmu-mir -155,mmu-mir -146a和TLR4 / MyD88 /NF-кB途径。

更新日期:2021-01-13
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