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To bulk or not to bulk? Intense genetic differentiation of seed dormancy among Stipa lagascae (Poaceae) genotypes accessed and multiplied in arid Tunisia
Restoration Ecology ( IF 2.8 ) Pub Date : 2021-01-12 , DOI: 10.1111/rec.13350
Marjolein Visser 1 , Amaury Beaugendre 1
Affiliation  

Many ecological restoration projects involving wild dryland grasses work with bulk-harvested seed, yet bulking may lead one to overlook and lose genetic variation that turns out to be crucial for restoration success, including specific dormancy breakdown requirements (conditional dormancy) and genetic variation in these. This article reports on the genetic variation of conditional seed dormancy of Stipa lagascae Roem. & Schult. (Poaceae), a winter-growing bunch grass with high-grazing value, native to the circum-Mediterranean areas but threatened with extinction. This grass is a cleistogamous inbreeder whose population remnants exhibit a fine-grained genetic structure in many ecological traits. Seed from different lineages was obtained at the end of a seed increase program that chose not to bulk seed during seed sourcing, seed increase, nor seed harvesting for final use. Over a period of 3 years, three germination trials were run with seed coming from 15 to 20 different seed lineages and harvested over different years in two related seed increase plots. Seed lineage was the single most important factor explaining differences in conditional dormancy. Seed weight did not add much to explain these differences. The singularity of behavior of each lineage was conserved over the different harvests. Young seed exhibits the strongest differentiation in conditional dormancy. To restore genetically diversified populations of this inbreeding grass, restoration practitioners should avoid to bulk seed during sourcing and seed increase, because unique seed lineages get easily lost unseen. However, seed for final use could be bulk harvested if seed is used when still conditionally dormant.

中文翻译:

批量还是不批量?在干旱的突尼斯获得和繁殖的针茅(禾本科)基因型之间种子休眠的强烈遗传分化

许多涉及野生旱地草的生态恢复项目使用大量收获的种子,但膨胀可能导致人们忽视和丢失对恢复成功至关重要的遗传变异,包括特定的休眠破坏要求(条件休眠)和这些中的遗传变异. 本文报道了针茅条件种子休眠的遗传变异罗姆。&舒尔特。(禾本科),一种冬季生长的束草,具有高放牧价值,原产于地中海沿岸地区,但面临灭绝威胁。这种草是一种闭锁近交种,其种群残余在许多生态特征中表现出细粒遗传结构。在种子增加计划结束时获得来自不同谱系的种子,该计划选择在种子采购、种子增加或最终使用的种子收获期间不散装种子。在 3 年的时间里,进行了 3 次发芽试验,种子来自 15 到 20 种不同的种子谱系,并在两个相关的种子增加地块中的不同年份收获。种子谱系是解释条件休眠差异的最重要因素。种子重量并没有增加太多来解释这些差异。每个谱系的行为的奇异性在不同的收获中得到了保护。年轻的种子在条件休眠中表现出最强的分化。为了恢复这种近交草的遗传多样性种群,恢复从业者应避免在采购和种子增加期间散装种子,因为独特的种子谱系很容易丢失而看不见。但是,如果在有条件的休眠时使用种子,则最终使用的种子可以大量收获。
更新日期:2021-01-12
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