The goal of this study was to analyse the effects of a protein‐deficient (PD) diet on antibody‐dependent cell‐mediated cytotoxicity (ADCC) in vitro against newborn larvae (NBL) of Trichinella spiralis in the lungs of infected rats. Two groups of weaning Wistar rats received a PD diet (6.5% casein) and other two received a control diet (C, 20% casein). After ten days, one group of each diet was infected (PD_I and C_I) with muscle larvae. Lung tissue extracts (LTE) and lung cell suspension (LCS) were obtained. PD_I had lower titres of anti‐NBL antibodies in LTE than C_I. In ADCC assays using control cells, NBL mortality percentage was lower with LTE from PD_I than LTE from C_I (P < .01). In assays using control cytotoxic sera, ADCC was exerted by LCS from C_I at all days post‐infection (p.i.), but only by LCS from 13 days p.i. from PD_I. ADCC assays combining LTE and LCS from the same group showed a lower response for PD_I than for C_I (P < .0001). LCS from PD_I contained lower numbers of neutrophils, eosinophils and FcεRI⁺ cells than C_I. PD may diminish ADCC activity against T spiralis NBL in lungs through alterations in specific antibodies and effector cells.

中文翻译：

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旋毛虫感染期间蛋白质缺乏会损害肺对新生幼虫的免疫力

本研究的目的是分析蛋白质缺乏 (PD) 饮食对体外抗体依赖性细胞介导的细胞毒性 (ADCC) 对感染大鼠肺部旋毛虫新生幼虫 (NBL) 的影响。两组断奶 Wistar 大鼠接受 PD 饮食（6.5% 酪蛋白），另外两组接受对照饮食（C，20% 酪蛋白）。十天后，每种饮食的一组被肌肉幼虫感染（PD _I和 C _I）。获得肺组织提取物（LTE）和肺细胞悬液（LCS）。PD_我曾在LTE抗NBL抗体比C低滴度_我。在使用对照细胞的 ADCC 测定中，来自 PD _{I 的}LTE 的NBL 死亡率百分比低于来自 C _{I 的}LTE( P  < .01)。在使用控制细胞毒性的血清测定，ADCC被选自C施加由LCS_我在所有天感染后（PI）从PD13天PI，而是仅由LCS_予。来自同一组的 LTE 和 LCS 组合的 ADCC 检测显示对 PD _{I 的}响应低于对 C _{I 的}响应( P  < .0001)。来自 PD _{I 的}LCS包含的中性粒细胞、嗜酸性粒细胞和 FcεRI ⁺细胞数量低于 C _I。PD 可能会通过改变特定抗体和效应细胞来降低 ADCC 对肺中T 螺旋体NBL 的活性。