Proteomic analysis of human follicular fluid from polycystic ovary syndrome patients,Biotechnology and Applied Biochemistry

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Proteomic analysis of human follicular fluid from polycystic ovary syndrome patients
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2021-01-13 , DOI: 10.1002/bab.2108
Young-Jin Sim ₁ , A-Reum Ryu _{2,

3} , Mi-Young Lee _{2,

3}

Affiliation

Comparative proteomic profiling of human follicular fluid (HFF) from polycystic ovary syndrome (PCOS) and non-PCOS patients who displayed low levels of fertility was carried out via two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry. HFF, an important reproductive fluid, was used for the proteomic analysis of PCOS patients to determine the effect of PCOS on folliculogenesis. HFF was obtained from 10 women (average age, 35 years) undergoing in vitro fertilization at two hospitals. The proteins of HFF were separated using 2-DE analysis and validated by Western blot assay. Approximately 250 protein spots were separated on the 2-DE gel. Among them, the expression levels of seven proteins were found to change at least 1.5-fold in the PCOS patient group. Three protein spots, albumin, uncharacterized protein 1, and uncharacterized protein 2, were downregulated in PCOS patients. However, four protein spots, gelsolin, vitamin D binding protein, serum albumin, and complement factor B, were upregulated in PCOS patient group. These proteins may serve as a panel of potential pathological biomarkers during fertilization and oocyte development.

中文翻译：

多囊卵巢综合征患者卵泡液的蛋白质组学分析

通过二维凝胶电泳 (2-DE) 与质谱法相结合，对多囊卵巢综合征 (PCOS) 和非 PCOS 患者生育能力低下的人卵泡液 (HFF) 进行了比较蛋白质组学分析。HFF 是一种重要的生殖液，用于 PCOS 患者的蛋白质组学分析，以确定 PCOS 对卵泡发生的影响。HFF 来自在两家医院接受体外受精的 10 名女性（平均年龄 35 岁）。使用 2-DE 分析分离 HFF 的蛋白质，并通过蛋白质印迹分析进行验证。在 2-DE 凝胶上分离出大约 250 个蛋白质点。其中，发现 PCOS 患者组中 7 种蛋白质的表达水平变化至少 1.5 倍。三个蛋白点，白蛋白，未鉴定蛋白 1，和未表征的蛋白质 2，在 PCOS 患者中下调。然而，在 PCOS 患者组中，凝溶胶蛋白、维生素 D 结合蛋白、血清白蛋白和补体因子 B 四个蛋白点上调。这些蛋白质可以作为受精和卵母细胞发育过程中一组潜在的病理生物标志物。

更新日期：2021-01-13

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