The development of biomaterials innovative compositions to be appropriate for hard tissue regeneration is vital and wished to improve the quality of life worldwide. In this work, using the pulsed laser deposition (PLD) technique, hydroxyapatite (HAP) was sputtered on a dense substrate of alumina at different times of exposure. The investigation of surface morphology indicated that grains of alumina were configured with dimensions of about 1.3–2.94 μm, while high content of porosity was observed. Moreover, the data revealed a significant plunge of surface roughness, whereas the average roughness decreased from 53 nm to 29 nm, and the maximum roughness valley depth decreased from 281 to 248 nm, recorded for 5 to 20 min of exposure time. The mechanical properties were examined non-destructively using ultrasonic waves, and it was noticed that the microhardness changed significantly from 24.7 ± 0.7 GPa to 27.2 ± 0.8 GPa for the compositions compared to 0 and 20 min samples. The attachment behavior of human osteoblasts cell line towards the obtained scaffolds was examined in vitro and prove that cells were proliferated and spread to cover the scaffold surface. This elucidates that manipulation of an innovative scaffold design can be executed based on tailoring of bioactive material (HAP) depositions on an inert biomaterial (alumina) to combine both mechanical and bioactivity, with less degradation rate.

开发适于硬组织再生的生物材料创新组合物至关重要，并希望改善全世界的生活质量。在这项工作中，使用脉冲激光沉积（PLD）技术，在不同的曝光时间将羟基磷灰石（HAP）溅射在致密的氧化铝基材上。表面形态的研究表明，氧化铝晶粒的尺寸约为1.3-2.94μm，而孔隙率却很高。此外，数据显示表面粗糙度明显下降，而平均粗糙度从53 nm降至29 nm，最大粗糙度谷深度从281 nm降至248 nm，记录了5至20分钟的曝光时间。使用超声波对机械性能进行了无损检测，并且注意到与0和20分钟样品相比，该组合物的显微硬度从24.7±0.7GPa显着变化到27.2±0.8GPa。在体外检查了人成骨细胞细胞系对获得的支架的附着行为，并证明细胞被增殖并扩散以覆盖支架表面。这阐明了可以基于对惰性生物材料（氧化铝）上生物活性材料（HAP）沉积物的定制来执行创新支架设计的操纵，以结合机械和生物活性，同时降低降解速率。在体外检查了人成骨细胞细胞系对获得的支架的附着行为，并证明细胞被增殖并扩散以覆盖支架表面。这阐明了可以基于对惰性生物材料（氧化铝）上生物活性材料（HAP）沉积物的定制来执行创新支架设计的操纵，以结合机械和生物活性，同时降低降解速率。在体外检查了人成骨细胞细胞系对获得的支架的附着行为，并证明细胞被增殖并扩散以覆盖支架表面。这阐明了可以基于对惰性生物材料（氧化铝）上生物活性材料（HAP）沉积物的定制来执行创新支架设计的操纵，以结合机械和生物活性，同时降低降解速率。