Immunostimulant and protective effects of Yarrowia lipolytica glucans against important pathogens, such as Escherichia coli, have not been investigated in goats and other ruminants. This study aimed to characterize Y. lipolytica N6-glucan (Yl-glucan) and its possible role in immunological signaling pathway activation and immunoprotection against E. coli in goat leukocytes. Characterization analyses showed that Y. lipolytica content had a mix of β and α-D-glucans, molecular weight of 3301.53 kDa and low solubility after the heat treatment. The stimulation of goat leukocytes with Yl-glucan induced protection against E. coli challenge. Remarkably, Yl-glucan and E. coli interaction increased gene expression of dectin-1 and TLR-2 receptors, signaling pathway Syk/NFκB, and cytokines, such as TNF-α and IL-10. As a consequence of signaling activation, phagocytosis, and nitric oxide production enhanced killing of pathogens. Altogether, Y. lipolytica-glucan demonstrated to possess an immunoprotective potential against E. coli through innate immune response modulation in goat leukocytes.

尚未在山羊和其他反刍动物中研究解脂耶氏酵母葡聚糖对重要病原体例如大肠杆菌的免疫刺激和保护作用。该研究旨在表征解脂耶氏酵母N6-葡聚糖（Y1-葡聚糖）及其在山羊白细胞中免疫信号途径激活和针对大肠杆菌的免疫保护中的可能作用。表征分析表明，解脂耶氏酵母含量具有β和α-D-葡聚糖的混合物，分子量为3301.53kDa，并且在热处理后溶解度低。Y1-葡聚糖刺激山羊白细胞诱导对大肠杆菌的保护挑战。值得注意的是，基-葡聚糖和大肠杆菌相互作用增加dectin-1的和TLR-2受体的基因表达，信号转导通路的Syk /NFκB，和细胞因子，如TNF-α和IL-10。信号激活的结果是，吞噬作用和一氧化氮的产生增强了对病原体的杀灭。总的来说，解脂耶氏酵母聚糖通过对山羊白细胞的先天免疫应答调节而具有针对大肠杆菌的免疫保护潜力。