Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M 20. We evaluated its performance for the detection of specific antibodies against multiple Leptospira serogroups in bovine. The microscopic agglutination test (MAT) was used as the gold standard. The performance of this ELISA was evaluated with a panel of sera (118 MAT confirmed positive and 97 MAT negative). The overall sensitivity was close to 85.6 % and the specificity was 83.5 %, according to the MAT reference method. Analytical specificity of the IgG-ELISA was evaluated using 50 bovine serum samples from animals showing serum antibodies against other pathogens that cause abortion in bovine, such as Brucella sp., Neospora sp. and Bovine Viral Diarrhea (BVD), and no cross-reaction was observed. This IgG-ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in bovine.

钩端螺旋体病是阿根廷的一种世界范围流行的人畜共患病，在低收入人群中是一个重大的公共卫生问题。牛钩端螺旋体病是造成牛群生产的严重经济问题，会导致流产，牛奶产量下降，犊牛死亡率降低以及日增重下降。我们开发了一种与声波钩端螺旋体血清群Icterohaemorrhagiae血清群Copenhageni M 20结合的酶联免疫吸附测定（ELISA）。我们评估了其对多种钩端螺旋体特异性抗体的检测性能。牛血清群。显微凝集试验（MAT）用作金标准。用一组血清（118 MAT确认阳性和97 MAT阴性）评估此ELISA的性能。根据MAT参考方法，总灵敏度接近85.6％，特异性为83.5％。使用来自动物的50个牛血清样品评估了IgG-ELISA的分析特异性，这些样品显示了针对会引起牛流产的其他病原体（例如布鲁氏菌属，新孢子虫属）的血清抗体。和牛病毒性腹泻（BVD），未观察到交叉反应。该IgG-ELISA可以替代MAT，以诊断牛的钩端螺旋体感染。