An six-week feeding trial was conducted to evaluate the effects of dietary n-3 polyunsaturated fatty acid (n-3 PUFA) lipid sources on growth performance, antioxidant capacity, tissue fatty acid profiles, and expression levels of genes involved in lipid metabolism of juvenile green mud crab (Scylla paramamosain) (initial weight 45.3 ± 0.07 g). Three isonitrogenous and isoenergetic experimental diets were formulated to contain fish oil (FO), krill oil (KO) and linseed oil (LO), respectively. The results indicated that crabs fed the KO diet had significantly higher percent weight gain (PWG), specific growth rate (SGR) and feed efficiency (FE) than those fed the LO diet, while no statistic differences were observed between KO and FO diets. Dietary FO could significantly increase the concentrations of malondialdehyde (MDA) and lipid hydroperoxide (LPO) in hemolymph and hepatopancreas compared to dietary KO and LO, similar results were found in the levels of hepatopancreas 8-hydroxy-2-deoxyguanosine (8-OHDG). Crabs fed the KO diet could significantly increase the activities of total superoxide dismutase (T-SOD) and catalase (CAT) in comparison with FO and LO diets. Crabs fed with FO and KO had significantly higher contents of 20:5n-3 (EPA) and 22:6n-3 (DHA) in hepatopancreas and muscle than those fed with LO. While dietary LO could significantly promote the accumulation of C18:3n-3 (ALA) in hepatopancreas and muscle. The anabolic pathway relevant genes: fas and 6 pg d were up-regulated in KO diet. The catabolic pathway relevant genes, hsl, was up-regulated in FO and KO diets, cpt1, was up-regulated in KO diet. Moreover, crabs fed diet supplemented with KO could significantly increase the expression levels of srb2, srebp1 and hnf4α. These findings will provide the reference for the development of the diet for the growth stage of S. paramamosain, and could contribute to deepen the understanding of the physiological metabolism of dietary fatty acids for S. paramamosain.

进行了为期六周的喂养试验，以评估膳食中的n-3多不饱和脂肪酸（n-3 PUFA）脂质来源对生长性能，抗氧化能力，组织脂肪酸谱以及参与脂质代谢的基因表达水平的影响。少年绿泥蟹（Sylla paramamosain）（初始重量45.3±0.07克）。配制了三种等氮和等能实验饲料，分别含有鱼油（FO），磷虾油（KO）和亚麻子油（LO）。结果表明，饲喂KO日粮的蟹比饲喂LO日粮的蟹的体重增加百分比（PWG），比生长速率（SGR）和饲料效率（FE）显着更高，而KO日粮和FO日粮之间没有统计学差异。与饮食KO和LO相比，饮食FO可以显着增加血淋巴和肝胰腺中丙二醛（MDA）和脂质过氧化氢（LPO）的浓度，在肝胰腺8-羟基-2-脱氧鸟苷（8-OHDG）的水平中也发现了类似的结果。与FO和LO饲料相比，用KO饲料喂养的螃蟹可以显着增加总超氧化物歧化酶（T-SOD）和过氧化氢酶（CAT）的活性。喂食FO和KO的螃蟹在肝胰腺和肌肉中的20：5n-3（EPA）和22：6n-3（DHA）含量明显高于喂LO的螃蟹。尽管饮食中的LO可以显着促进肝胰腺和肌肉中C18：3n-3（ALA）的积累。合成代谢途径相关基因：KO饮食中fas和6 pg d上调。分解代谢途径相关基因hsl在FO和KO日粮中上调，cpt1在KO日粮中上调。此外，喂食添加了KO的日粮的螃蟹可以显着提高srb2，srebp1和hnf4α的表达水平。这些研究结果将为副沙门菌的生长阶段饮食的发展提供参考，并有助于加深对副沙门菌的饮食脂肪酸生理代谢的认识。