Resveratrol, a potent antioxidant, can be an alternative semen extender constituent to protect spermatozoa against reactive oxygen species (ROS); however, effects on sperm quality post-thawing and sperm function is not well understood. This study, therefore, was conducted to investigate effects of resveratrol supplementation to semen extender on sperm quality post-thawing. Bull semen was cryopreserved using extenders not supplemented or supplemented with 0.05, 0.1, or 1 mM resveratrol. Supplementation of extender with resveratrol at 0.05 mM resulted in greater (P < 0.05) sperm progressive motility, average path velocity, straight linear velocity, linearity and straightness when compared with no or 1 mM supplementations. Furthermore, effects of 0.05 mM resveratrol supplementations on plasma membrane and acrosome integrity and sperm fertilization capacity using in vitro procedures were investigated. Supplementation of semen extender with resveratrol resulted in a greater (P < 0.05) proportion of frozen-thawed spermatozoa with an intact acrosome and plasma membrane. Results from in vitro fertilization studies indicated there were no differences (P> 0.05) when there was no supplementation or supplementation with 0.05 mM resveratrol on embryo development to the cleavage and blastocyst stages. In conclusion, addition of resveratrol to bull semen extender resulted in greater sperm quality post-thawing in a dose-dependent manner, with values for variables related to sperm quality being greater when there was resveratrol supplementation at the 0.05 mM concentration. Proportion of embryo developing to the cleavage and blastocyst stages after in vitro fertilization was not affected by resveratrol supplementation to semen extenders.

白藜芦醇是一种有效的抗氧化剂，可以作为替代的精液补充剂成分来保护精子免受活性氧 (ROS) 的侵害；然而，解冻后对精子质量和精子功能的影响尚不清楚。因此，本研究旨在研究在精液稀释剂中添加白藜芦醇对解冻后精子质量的影响。公牛精液使用未添加或添加 0.05、0.1 或 1 mM 白藜芦醇的稀释剂进行冷冻保存。用 0.05 mM 的白藜芦醇补充增量剂导致更大的 ( P< 0.05) 与不添加或 1 mM 补充剂相比，精子进行性运动、平均路径速度、直线速度、线性度和直线度。此外，使用体外程序研究了 0.05 mM 白藜芦醇补充剂对质膜和顶体完整性以及精子受精能力的影响。用白藜芦醇补充精液稀释剂导致具有完整顶体和质膜的冻融精子比例更高（P < 0.05）。体外受精研究结果表明没有差异（P> 0.05) 当胚胎发育到卵裂和囊胚阶段时没有补充或补充 0.05 mM 白藜芦醇。总之，将白藜芦醇添加到公牛精液稀释剂中会以剂量依赖的方式在解冻后提高精子质量，当添加 0.05 mM 浓度的白藜芦醇时，与精子质量相关的变量值更大。体外受精后胚胎发育到卵裂和囊胚阶段的比例不受向精液补充剂补充白藜芦醇的影响。