Abstract—

Lignin is one of the most abundant biopolymers. Information about the functional composition and structure of various lignins may be useful in the study of biosynthesis processes in plants. The subject of the article is the determination of ³¹P NMR spectroscopy possibilities to identify lignins obtained from various plant forms. To obtain spectra on ³¹P nuclei, a modification of the studied samples was applied by phosphitylating their OH-groups with a special reagent. We obtained qualitative and quantitative analysis of samples of softwood and hardwood lignins and herbaceous plants. According to the NMR spectra we noted the differences in composition and structure of the studied lignins. We confirmed that the structure of lignins of softwood and hardwood wood mainly contain guaiacilpropane and syringylpropane structural units, respectively. We found that lignins of herbaceous plants contain all types of OH-groups characteristic for lignins, including p-hydroxyphenyl groups. It was observed that samples of herbaceous plants lignins contain fragments of flavonoid structures in most cases. The ³¹P NMR spectrum of quercetin, as the flavonoids representative, was registered and also modeled using the ACDLabs software package to confirm the presence of flavone structures in the macromolecules of the herbaceous lignins.

中文翻译：

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31 P NMR光谱法比较各种植物形式的木质素

摘要—

木质素是最丰富的生物聚合物之一。关于各种木质素的功能组成和结构的信息可能对研究植物的生物合成过程有用。本文的主题是确定³¹ P NMR光谱法鉴定从各种植物形式获得的木质素的可能性。在³¹上获得光谱P核是对研究样品的修饰，方法是使用特殊试剂将其羟基进行磷酸化。我们获得了软木和硬木木质素以及草本植物样品的定性和定量分析。根据NMR光谱，我们注意到所研究的木质素在组成和结构上的差异。我们证实，软木和硬木木质素的结构分别主要包含愈创木丙烷和丁香基丙烷结构单元。我们发现，草本植物的木质素包含木质素特有的所有类型的OH-基团，包括对羟基苯基。据观察，在大多数情况下，草本植物木质素样品含有类黄酮结构的片段。在³¹登记槲皮素的P NMR光谱，以类黄酮为代表，并使用ACDLabs软件包进行建模，以确认草本木质素大分子中黄酮结构的存在。