Abstract

The goal of the study was to develop a complex technique for the extraction and purification of polysaccharides from the leaves of birch (Betula pendula Roth., Betula pubescens Ehrh., family Betulaceae) (PSfB). The following characteristics have been used as criteria for estimating the effect of the parameters studied on the production of target compounds: the yield of PSfB (the gravimetric method), the protein content (spectrophotometry), the molecular weight distribution (high performance size-exclusion chromatography), and the degree of purification from low-molecular-weight impurities (LMWI). The following optimal parameters have been determined in experiments at different stages of PSfB production: the degree of grinding of a raw material 1.2–3 mm; the pH value of the extractant (purified water with the pH value of 6.5–7.0); the raw material : extractant ratio 1 : 20; the extraction temperature 50°C; the temperature and the degree of evaporation 50°C, four times; the concentrate : ethanol ratio 1 : 3; and the method of purification from LMWI by ultrafiltration. As a result of the work, an optimized method for obtaining PSfB has been developed, which affords a high yield without the loss of quality of the product (minimal admissible content of LMWI with the maximum content of high-molecular-weight fractions). The method is the basis for the development of laboratory regulations for obtaining an active pharmaceutical substance based on polysaccharides from birch leaves.

中文翻译：

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从白桦叶中获得多糖的技术的发展（Betula pendula Roth。和Betula pubescens Ehrh。）

摘要

该研究的目的是开发一种复杂的技术，用于从桦树叶片中提取和纯化多糖（Betula pendula Roth。，Betula pubescensEhrh。，家庭（科）（PSfB）。以下特征已用作评估所研究参数对目标化合物生产的影响的标准：PSfB的产率（重量法），蛋白质含量（分光光度法），分子量分布（高效尺寸排阻）色谱）以及从低分子量杂质（LMWI）纯化的程度。在PSfB生产的不同阶段的实验中，确定了以下最佳参数：原料的研磨度为1.2–3 mm；萃取剂的pH值（pH值为6.5-7.0的纯净水）；原料：萃取剂比例为1：20；提取温度50℃；温度和蒸发度50 °C，四次；浓缩液：乙醇的比例为1：3；以及通过超滤从LMWI中纯化的方法。这项工作的结果是，开发了一种获得PSfB的优化方法，该方法可提供高收率而又不降低产品质量（LMWI的最低容许含量，而高分子量组分的含量最高）。该方法是制定实验室规章的基础，该规章旨在从桦树叶片中获得基于多糖的活性药物。