β-cyclodextrin (β-CD) is biocompatible and biodegradable polymer having inherent hydrophobic cavities, thus is widely used as a drug nanocarrier for less water soluble drugs. Naringin is a pharmacologically active important compound however, its therapeutic efficacy is limited due to its unique physicochemical properties including lower aqueous solubility. This study reports improvement in bactericidal potentials of naringin through construction of its nano-formulation using biocompatible β-CD. Naringin loaded β-CD NPs were prepared via solvent evaporation method and characterized using various techniques such as FTIR, UV–visible spectroscopy, AFM, DSC and TGA analysis. Tetrazolium Microplate and AFM assays were used for investigating the bactericidal potential of naringin loaded β-CD NPs. Monodispersed NPs with spherical morphology of 70.00 ± 15.06 nm mean diameter were obtained that possessed net negative surface charge of − 18.20 ± 3.24 mV and were highly stable and did not change the chemical nature of the drug in various media. Antibacterial activity of naringin in β-CD NPs significantly enhanced that was authenticated by AFM, showing prominent destructive changes in the bacterial cell’s morphology through destabilization of outer membrane of bacterial cells by drug loaded NPs. Results of this study suggest that the β-CD NPs of naringin could be used as a successful formulation for developing more efficacious form of the drug against various bacterial infections.

β-环糊精（β-CD）是具有固有疏水腔的生物相容性和可生物降解的聚合物，因此被广泛用作水溶性较小的药物的药物纳米载体。柚皮苷是具有药理活性的重要化合物，但是，由于其独特的物理化学性质（包括较低的水溶性），其治疗功效受到限制。这项研究报告说，通过使用生物相容性β-CD构建其柚皮素纳米制剂，可以改善柚皮素的杀菌潜力。通过溶剂蒸发法制备负载柚木素的β-CDNP，并使用多种技术进行表征，例如FTIR，紫外可见光谱，AFM，DSC和TGA分析。使用四氮唑微孔板和AFM测定法来研究带有柚皮苷的β-CDNP的杀菌潜力。球形形态为70.00±15的单分散NP。获得的平均直径为06 nm，具有-18.20±3.24 mV的净负表面电荷，并且高度稳定并且在各种介质中均未改变药物的化学性质。柚皮苷在β-CDNPs中的抗菌活性得到AFM的证实，显着增强，通过载药的NPs使细菌细胞外膜失稳，显示出细菌细胞形态的显着破坏性变化。这项研究的结果表明，柚皮苷的β-CDNPs可以作为开发更有效形式的抗各种细菌感染药物的成功制剂。柚皮苷在β-CDNPs中的抗菌活性得到AFM的证实，显着增强，通过载药的NPs使细菌细胞外膜失稳，显示出细菌细胞形态的显着破坏性变化。这项研究的结果表明，柚皮苷的β-CDNPs可以作为开发更有效形式的抗各种细菌感染药物的成功制剂。柚皮苷在β-CDNPs中的抗菌活性得到AFM的证实，显着增强，通过载药的NPs使细菌细胞外膜失稳，显示出细菌细胞形态的显着破坏性变化。这项研究的结果表明，柚皮苷的β-CDNPs可以作为开发更有效形式的抗各种细菌感染药物的成功制剂。