This study aimed to evaluate the toxic action of methyl eugenol against Drosophila melanogaster and to evaluate the myorelaxant effect of this constituent on bronchioles isolated from swine from the Sus scrofa domesticus species. The toxicity assay was performed using the fumigation method while myorelaxant activity was assessed by the administration of increasing and cumulative concentrations (1–1000 μM) to swine bronchial rings under 1 gF tension in an organ bath precontracted with 60 mM potassium chloride (KCl) and 10 μM acetylcholine (ACh). The results showed that methyl eugenol possessed insecticidal action (LC₅₀ 0.116 μg/mL of air). The compound showed a myorelaxant activity over bronchioles (IC₅₀ of 25.50±14.93 μM and 12.79±9.57 μM for KCl and ACh, respectively). This study serves as starting point for further detailed studies focusing on both the insecticidal and bronchodilatory activity of methyl eugenol.

这项研究旨在评估甲基丁香酚对果蝇果蝇的毒性作用，并评估该成分对猪（Sus scrofa domesticus）分离出的细支气管的髓鞘舒张作用。使用熏蒸法进行毒性试验，同时通过在预先用60 mM氯化钾（KCl）和乙草胺预先包埋的器官浴中，在1 gF张力下对猪支气管环施用递增和累积的浓度（1-1000μM）来评估肌无松弛活性。 10μM乙酰胆碱（ACh）。结果表明，甲基丁香酚具有杀虫作用（LC _50为0.116μg/ mL空气）。该化合物相对于细支气管显示出肌肉松弛活性（IC ₅₀对于KCl和ACh，分别为25.50±14.93μM和12.79±9.57μM）。该研究作为进一步详细研究的起点，侧重于甲基丁香酚的杀虫和支气管扩张活性。