Newcastle disease virus is a member of family Paramyxoviridae that infects chicken. Its genome comprises ~15.2 kb negative-sense RNA that encodes six major proteins. The virus encodes various proteins; among all, nucleocapsid (NP) and matrix (M) help in virus replication and its budding from the host cells, respectively. In this study, we investigated the intracellular distribution of NP and M upon expression in the yeast Saccharomyces cerevisiae. We observed nuclear targeting of M, and vacuolar localization of NP was observed in a fraction of yeast cells. Prolonged expression of GFP fused NP or M resulted in altered cell viability and intracellular production of reactive oxygen species in yeast cells. The expression of viral proteins did not alter the morphology and number of the organelles such as nucleus, mitochondria, endoplasmic reticulum, and peroxisomes. However, a significant effect was observed on vacuolar morphology and number in yeast cells. These observations point towards the importance of host cellular reorganization in viral infection. These findings may enable us to understand the conserved pathways affected in eukaryotic cells as a result of viral protein expression.

新城疫病毒是感染鸡的副粘病毒科的成员。它的基因组包含约 15.2 kb 的负义 RNA，编码六种主要蛋白质。该病毒编码各种蛋白质；其中，核衣壳 (NP) 和基质 (M) 分别有助于病毒复制及其从宿主细胞中出芽。在这项研究中，我们研究了 NP 和 M 在酵母Saccharomyces cerevisiae中表达后的细胞内分布. 我们观察到 M 的核靶向，并且在一部分酵母细胞中观察到 NP 的液泡定位。GFP 融合的 NP 或 M 的延长表达导致酵母细胞中细胞活力和细胞内活性氧种类的改变。病毒蛋白的表达并没有改变细胞器的形态和数量，例如细胞核、线粒体、内质网和过氧化物酶体。然而，观察到对酵母细胞中液泡形态和数量的显着影响。这些观察表明宿主细胞重组在病毒感染中的重要性。这些发现可能使我们能够了解由于病毒蛋白表达而在真核细胞中影响的保守途径。