Cudrania tricuspidata (C. tricuspidata) is widespread throughout Asia and has known to have various physiological activities such as, inflammation, diabetes, obesity and tumor. Cudrania tricuspidata, a rich source of xanthones and flavonoids, have been investigated phytochemically and biologically. However, research of these compounds on platelets is limited. Therefore, we searched for a new substance from various xanthones and flavonoids in C. tricuspidata. We confirmed the results that steppogenin and isoderrone suppress human platelets among the various components isolated from C. tricuspidata, and as a result of analyzing the antiplatelet effect using additional new samples, we found that cudraxanthone B (CXB) has the effect of suppressing human platelets. Therefore, we studied the potential efficacies of CXB on human platelet aggregation and its inhibitory mechanism. Inhibitory effects of CXB on platelet aggregation were assessed using washed platelets, followed by measurement of [Ca2+]i mobilization and dense granule release, fibrinogen binding, fibronectin adhesion assay, and clot retraction. Our data showed that CXB suppressed collagen-induced human platelet aggregation, [Ca2+]i mobilization, fibrinogen binding, fibronectin adhesion and clot retraction without cytotoxicity. Thus, our results show that inhibitory effects of CXB on human platelet activation and thrombus formation, suggesting its potential use as a natural substance for preventing platelet-induced thrombosis.

中文翻译：

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头孢蒽酮B的抗血小板作用与抑制钙动员，αIIbβ3活化和血凝块收缩有关

中华（Cdrania tricuspidata）（C. tricuspidata）遍布整个亚洲，已知具有各种生理活动，例如炎症，糖尿病，肥胖症和肿瘤。dra草（Cdrania tricuspidata）是黄原酮和类黄酮的丰富来源，已经在植物化学和生物学上进行了研究。然而，这些化合物在血小板上的研究是有限的。因此，我们从C. tricuspidata中的各种氧杂蒽酮和类黄酮中寻找一种新物质。我们证实了这一结果，即steppogenin和isoderrone抑制了从C. tricuspidata分离的各种成分中的人血小板，并且通过使用其他新样品分析抗血小板作用的结果，我们发现草酮B（CXB）具有抑制人血小板的作用。 。因此，我们研究了CXB对人血小板聚集的潜在功效及其抑制机制。使用洗涤过的血小板评估CXB对血小板聚集的抑制作用，然后测量[Ca2 +] i动员和致密颗粒释放，纤维蛋白原结合，纤连蛋白粘附测定和血凝块收缩。我们的数据显示CXB抑制胶原蛋白诱导的人类血小板聚集，[Ca2 +] i动员，纤维蛋白原结合，纤连蛋白粘附和血凝块收缩而无细胞毒性。因此，我们的结果表明，CXB对人体血小板活化和血栓形成具有抑制作用，表明其潜在用途作为预防血小板诱导的血栓形成的天然物质。然后测量[Ca2 +] i动员和致密颗粒释放，纤维蛋白原结合，纤连蛋白粘附测定和血凝块收缩。我们的数据显示CXB抑制胶原蛋白诱导的人类血小板聚集，[Ca2 +] i动员，纤维蛋白原结合，纤连蛋白粘附和血凝块收缩而无细胞毒性。因此，我们的结果表明，CXB对人体血小板活化和血栓形成具有抑制作用，表明其潜在用途作为预防血小板诱导的血栓形成的天然物质。然后测量[Ca2 +] i动员和致密颗粒释放，纤维蛋白原结合，纤连蛋白粘附测定和血凝块收缩。我们的数据显示CXB抑制胶原蛋白诱导的人类血小板聚集，[Ca2 +] i动员，纤维蛋白原结合，纤连蛋白粘附和血凝块收缩而无细胞毒性。因此，我们的结果表明，CXB对人体血小板活化和血栓形成具有抑制作用，表明其潜在用途作为预防血小板诱导的血栓形成的天然物质。