Preparation and chromatographic evaluation of the hydrophilic interaction chromatography stationary phase based on nucleosides or nucleotides,Analytical Methods

当前位置： X-MOL 学术 › Anal. Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Preparation and chromatographic evaluation of the hydrophilic interaction chromatography stationary phase based on nucleosides or nucleotides
Analytical Methods ( IF 2.7 ) Pub Date : 2020-12-17 , DOI: 10.1039/d0ay02016h
Tingfeng Tang _{1,

2,

3,

4} , Dandan Guo _{1,

2,

3,

4} , Shaohua Huang _{1,

2,

3,

4,

5}

Affiliation

In this work, a series of novel hydrophilic interaction chromatography (HILIC) stationary phases were prepared by grafting nucleosides or nucleotides on the surface of silica gel. Firstly, the silica was modified with 3-glycidoxypropyltrimethoxysilane (GPTMS). And then nucleosides or nucleotides were bonded on the surface of GPTMS-modified silica through the epoxy–amine ring-opening reaction to provide four HILIC materials. These obtained stationary phases were successfully characterized by Fourier transform-infrared spectroscopy (FT-IR) and elemental analysis (EA), respectively. Effects of column temperature, water content of the mobile phase, pH and buffer concentration on the retention behavior of these HILIC materials and the corresponding separation mechanism were evaluated using various nucleosides and nucleobases, respectively. In addition, polar and hydrophilic compounds such as amino acids and water-soluble vitamins were successfully separated using the corresponding columns, showing application potential for the separation of bioactive substances.

中文翻译：

基于核苷或核苷酸的亲水作用色谱固定相的制备及色谱评价

在这项工作中，通过将核苷或核苷酸接枝到硅胶表面制备了一系列新型的亲水相互作用色谱（HILIC）固定相。首先，用3-环氧丙氧基丙基三甲氧基硅烷（GPTMS）对二氧化硅进行改性。然后，核苷或核苷酸通过环氧-胺开环反应键合在GPTMS改性的二氧化硅表面上，从而提供了四种HILIC材料。这些获得的固定相分别通过傅里叶变换红外光谱（FT-IR）和元素分析（EA）进行了成功表征。分别使用各种核苷和核碱基评估了柱温，流动相中的水含量，pH和缓冲液浓度对这些HILIC材料的保留行为和相应的分离机理的影响。此外，

更新日期：2021-01-12

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11