This work presents a new procedure to synthesize ruthenium–phthalocyanine complexes and uses diverse spectroscopic techniques to characterize trans-[RuCl(Pc)DMSO] (I) (Pc = phthalocyanine) and trans-[Ru(Pc)(4-ampy)₂] (II) (4-ampy = 4-aminopyridine). The triplet excited-state lifetimes of (I) measured by nanosecond transient absorption showed that two processes occurred, one around 15 ns and the other around 3.8 μs. Axial ligands seemed to affect the singlet oxygen quantum yield. Yields of 0.62 and 0.14 were achieved for (I) and (II), respectively. The lower value obtained for (II) probably resulted from secondary reactions of singlet oxygen in the presence of the ruthenium complex. We also investigate how axial ligands in the ruthenium–phthalocyanine complexes affect their photo-bioactivity in B16F10 murine melanoma cells. In the case of (I) at 1 μmol/L, photosensitization with 5.95 J/cm² provided B16F10 cell viability of 6%, showing that (I) was more active than (II) at the same concentration. Furthermore, (II) was detected intracellularly in B16F10 cell extracts. The behavior of the evaluated ruthenium–phthalocyanine complexes point to the potential use of (I) as a metal-based drug in clinical therapy. Changes in axial ligands can modulate the photosensitizer activity of the ruthenium phthalocyanine complexes.

这项工作提出了一种合成钌-酞菁配合物的新方法，并使用了多种光谱技术来表征 反式[RuCl（Pc）DMSO] （一世） （Pc =酞菁）和 反式-[Ru（Pc）（4-ampy）₂ ]（二）（4-ampy ＝ 4-氨基吡啶）。的三重态激发态寿命为（一世）通过纳秒瞬态吸收进行的测量显示，发生了两个过程，一个过程在15 ns左右，另一个过程在3.8μs左右。轴向配体似乎影响单线态氧量子产率。的收率分别为0.62和0.14（一世） 和 （二）， 分别。获得的较低值（二）可能是由于钌络合物存在下单重态氧的二次反应所致。我们还研究了钌-酞菁配合物中的轴向配体如何影响其在B16F10鼠黑色素瘤细胞中的光生物活性。如果是（一世）在1μmol/ L下，以5.95 J / cm ^2的光敏作用可提供6％的B16F10细胞活力，表明（一世） 比 （二）在相同的浓度。此外，（二）在B16F10细胞提取物中的细胞内检测到了BMP。经评估的钌-酞菁配合物的行为表明了其潜在用途。（一世）作为临床治疗中的金属基药物。轴向配体的变化可以调节钌酞菁配合物的光敏剂活性。