Introduction: The endoplasmic reticulum transmembrane proteins Sec61, Sec62, and Sec63 are responsible for the intracellular trafficking of precursor proteins and affect intracellular signaling. SEC62 overexpression has been linked to various human cancers. Our aim was to investigate SEC62 and SEC63 expression in hepatocellular carcinoma (HCC) and surrounding liver tissue. Patients and Methods: Primary liver tissue was collected from 11 consecutive patients (70 ± 9 years; 10 men) who underwent HCC resection. In the HCC and the tumor-surrounding liver tissue we investigated SEC62 und SEC63 mRNA expression using quantitative real-time PCR. For Sec62, immunohistochemistry was performed. Results: SEC62and SEC63 total mRNA contents were significantly (p = 0.001) higher in HCCs (C_T 22.5 ± 0.4 and 22.6 ± 0.3) when compared to the surrounding tissue (C_T 24.6 ± 0.6 and 25.1 ± 0.9). Using the comparative C_Tmethod, SEC62 and SEC63 expression in HCC was increased 5- and 8.1-fold, respectively, in comparison to surrounding tissue. For Sec62 immunohistochemistry, the mean immunoreactive scores (IRS) were 7.9 ± 2.9 for HCC and 4.8 ± 1.2 for non-tumorous liver (p = 0.027). The mean IRS in HCC were 5.7 ± 3.5 and 8.9 ± 2.3 for patients without (n = 3) and with tumor recurrence (n = 8), respectively. Conclusions: Overexpression of SEC62 and SEC63 is a common feature of HCC. The role of Sec62 as a prognostic marker for tumor recurrence after surgery and its potential role in treatment stratification must be addressed in future studies.
Visc Med

中文翻译：

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SEC62和SEC63在肝细胞癌和肿瘤周围肝组织中的表达

简介：内质网跨膜蛋白Sec61，Sec62和Sec63负责细胞内前体蛋白的运输并影响细胞内信号传导。SEC62过表达与多种人类癌症有关。我们的目的是研究肝细胞癌（HCC）和周围肝组织中SEC62和SEC63的表达。患者和方法：从11例接受HCC切除的连续患者（70±9岁； 10名男性）中收集原发性肝组织。在HCC和肿瘤周围的肝组织中，我们研究了SEC62和SEC63使用定量实时PCR进行mRNA表达。对于Sec62，进行了免疫组织化学。结果： 与周围组织相比（C _T 24.6±0.6和25.1±0.9），HCCs（C _T 22.5±0.4和22.6±0.3）中SEC62和SEC63的总mRNA含量显着更高（p = 0.001 ）。使用比较C _T方法，与周围组织相比，HCC中SEC62和SEC63的表达分别增加了5倍和8.1倍。对于Sec62免疫组织化学，HCC的平均免疫反应评分（IRS）为7.9±2.9，非肿瘤肝的平均免疫反应评分（IRS）为4.8±1.2（p= 0.027）。无（n = 3）和有肿瘤复发（n = 8）的患者的HCC平均IRS分别为5.7±3.5和8.9±2.3 。结论： SEC62和SEC63的过表达是HCC的共同特征。Sec62作为手术后肿瘤复发的预后标志物的作用及其在治疗分层中的潜在作用必须在未来的研究中加以探讨。
Visc Med