Glucose is a major energy fuel for the brain, however, less is known about specificities of its metabolism in distinct cerebral areas. Here we examined the regional differences in glucose utilization between the hypothalamus and hippocampus using in vivo indirect ¹³C magnetic resonance spectroscopy (¹H-[¹³C]-MRS) upon infusion of [1,6-¹³C₂]glucose. Using a metabolic flux analysis with a 1-compartment mathematical model of brain metabolism, we report that compared to hippocampus, hypothalamus shows higher levels of aerobic glycolysis associated with a marked gamma-aminobutyric acid-ergic (GABAergic) and astrocytic metabolic dependence. In addition, our analysis suggests a higher rate of ATP production in hypothalamus that is accompanied by an excess of cytosolic nicotinamide adenine dinucleotide (NADH) production that does not fuel mitochondria via the malate-aspartate shuttle (MAS). In conclusion, our results reveal significant metabolic differences, which might be attributable to respective cell populations or functional features of both structures.

中文翻译：

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[1,6-13C2]葡萄糖的体内代谢显示小鼠海马和下丘脑之间不同的神经能功能。

葡萄糖是大脑的主要能源，然而，人们对它在不同大脑区域的代谢特异性知之甚少。在这里，我们通过注入[1,6- ¹³ C ₂ ]后使用体内间接¹³ C磁共振波谱（¹ H- [ ¹³ C] -MRS）检查了下丘脑和海马之间葡萄糖利用的区域差异。]葡萄糖。使用具有脑室代谢的1室数学模型的代谢通量分析，我们报告与海马相比，下丘脑显示出更高水平的需氧糖酵解，并伴有明显的γ-氨基丁酸-能（GABAergic）和星形细胞代谢依赖性。此外，我们的分析表明，下丘脑中ATP产生的速率更高，同时伴随着过量的胞质烟酰胺腺嘌呤二核苷酸（NADH）产生，而苹果酸-天冬氨酸穿梭（MAS）不能为线粒体提供燃料。总之，我们的结果显示出明显的代谢差异，这可能归因于各自的细胞群或两种结构的功能特征。