Plant-derived exosome-like nanovesicles (EPDENs) have recently been isolated and evaluated as potential bioactive nutraceutical biomolecules. It has been hypothesized that EPDENs may exert their activity on mammalian cells through their specific cargo. In this study, we isolated and purified EPDENs from the strawberry juice of Fragaria x ananassa (cv. Romina), a new cultivar characterized by a high content of anthocyanins, folic acid, flavonols, and vitamin C and an elevated antioxidant capacity. Fragaria-derived EPDENs were purified by a series of centrifugation and filtration steps. EPDENs showed size and morphology similar to mammalian extracellular nanovesicles. The internalization of Fragaria-derived EPDENs by human mesenchymal stromal cells (MSCs) did not negatively affect their viability, and the pretreatment of MSCs with Fragaria-derived EPDENs prevented oxidative stress in a dose-dependent manner. This is possibly due to the presence of vitamin C inside the nanovesicle membrane. The analysis of EPDEN cargo also revealed the presence of small RNAs and miRNAs. These findings suggest that Fragaria-derived EPDENs may be considered nanoshuttles contained in food, with potential health-promoting activity.

中文翻译：

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草莓衍生的外泌体样纳米颗粒可防止人间质基质细胞的氧化应激

植物来源的外来体样纳米囊泡（EPDENs）最近已被分离出来，并被评估为潜在的生物活性营养生物分子。据推测，EPDENs可能通过其特定的货物在哺乳动物细胞上发挥其活性。在这项研究中，我们从草莓属植物Fragaria x ananassa（cv。Romina）的草莓汁中分离和纯化了EPDENs ，该新品种的特征在于花青素，叶酸，黄酮醇和维生素C含量高，抗氧化能力增强。通过一系列离心和过滤步骤纯化了草莓来源的EPDEN。EPDENs显示出与哺乳动物细胞外纳米囊泡相似的大小和形态。草莓的内部化间充质基质细胞（MSCs）衍生的EPDENs不会对其生存能力产生负面影响，并且用草莓属EPDENs预处理MSCs以剂量依赖的方式阻止了氧化应激。这可能是由于纳米囊泡膜中存在维生素C。对EPDEN货物的分析还揭示了小RNA和miRNA的存在。这些发现表明，来自草莓属的EPDENs可以被认为是食品中所含的纳米穿梭巴士，具有促进健康的潜能。