Inflammation and oxidative stress are recognized as important contributors of brain injury in newborns due to a perinatal hypoxic-ischemic (HI) insult. Genetic variability in these pathways could influence the response to HI and the outcome of brain injury. The aim of our study was to evaluate the impact of common single-nucleotide polymorphisms in the genes involved in inflammation and response to oxidative stress on brain injury in newborns after perinatal HI insult based on the severity and pattern of magnetic resonance imaging (MRI) findings. The DNA of 44 subjects was isolated from buccal swabs. Genotyping was performed for NLRP3 rs35829419, CARD8 rs2043211, IL1B rs16944, IL1B rs1143623, IL1B rs1071676, TNF rs1800629, CAT rs1001179, SOD2 rs4880, and GPX1 rs1050450. Polymorphism in CARD8 was found to be protective against HI brain injury detected by MRI overall findings. Polymorphisms in IL1B were associated with posterior limb of internal capsule, basal ganglia, and white matter brain patterns determined by MRI. Our results suggest a possible association between genetic variability in inflammation- and antioxidant-related pathways and the severity of brain injury after HI insult in newborns.

中文翻译：

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CARD8和IL1B多态性影响低温治疗的新生儿缺氧缺血性脑病的MRI脑模式

由于围产期缺氧缺血（HI）损伤，炎症和氧化应激被认为是新生儿脑损伤的重要原因。这些途径的遗传变异性可能会影响对HI的反应和脑损伤的结果。我们研究的目的是根据磁共振成像（MRI）的严重程度和模式，评估围产期HI损伤后新生儿炎症和氧化应激反应基因中常见的单核苷酸多态性对新生儿脑损伤的影响。从口腔拭子中分离出44名受试者的DNA。对NLRP3 rs35829419，CARD8 rs2043211，IL1B rs16944，IL1B rs1143623，IL1B执行了基因分型rs1071676，TNF rs1800629，CAT rs1001179，SOD2 rs4880和GPX1 rs1050450。通过MRI总体发现，发现CARD8中的多态性可预防HI脑损伤。IL1B中的多态性与MRI确定的内囊后肢，基底神经节和白质脑模式有关。我们的结果表明，炎症和抗氧化剂相关途径的遗传变异与新生儿HI损伤后脑损伤的严重程度之间可能存在关联。