Introduction
This study investigated the protective effects of antcin C against cerebral haemorrhage injury.

Material and methods
Cerebral haemorrhage was treated with antcin C 100 mg/kg i.p. at 60 min after the induction of cerebral injury. Neurological scores and volumes of cerebral injury were assessed to determine the effects of antcin C, based on oxidative stress and serum mediators of inflammation by ELISA. qRT-PCR was used to estimate the mRNA expression of Toll-like receptor 4 (TLR-4) and interleukin-1 receptor-associated kinase 4 (IRAK4) proteins in the cerebral tissue of rats with cerebral haemorrhage. Western blot assay and histopathology were also performed.

Results
The findings suggest that treatment with antcin C reduced the neurological scores and volumes of cerebral injury in cerebral injured rats. Parameters of oxidative stress and cytokine levels were reduced in the serum of the antcin C-treated group compared with the negative control group. Treatment with antcin C ameliorated the expression of TLR-4, IRAK4, and zonula occludens-1 (ZO-1) proteins in the cerebral tissue of cerebral injured rats.

Conclusions
The results revealed that treatment with antcin C protected against cerebral haemorrhage damage by controlling microglia inflammation through the TLR-4 pathway.



中文翻译：

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Antcin C通过抑制TLR-4通路改善脑出血引起的神经元炎症

引言
本研究调查了antcin C 对脑出血损伤的保护作用。

材料和方法
在诱导脑损伤后 60 分钟，用 antcin C 100 mg/kg ip 治疗脑出血。基于氧化应激和炎症的血清介质，通过ELISA评估神经学评分和脑损伤体积以确定antcin C的作用。qRT-PCR用于估计脑出血大鼠脑组织中Toll样受体4（TLR-4）和白细胞介素1受体相关激酶4（IRAK4）蛋白的mRNA表达。还进行了蛋白质印迹测定和组织病理学。

结果
研究结果表明，用antcin C治疗降低了脑损伤大鼠的神经系统评分和脑损伤体积。与阴性对照组相比，antcin C 治疗组血清中氧化应激参数和细胞因子水平降低。antcin C 治疗改善了脑损伤大鼠脑组织中 TLR-4、IRAK4 和 zonula occludens-1 (ZO-1) 蛋白的表达。

结论
结果表明，antcin C 治疗可通过 TLR-4 通路控制小胶质细胞炎症，从而防止脑出血损伤。