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Cognitive enhancement and neuroprotective effects of a traditional Chinese herbal compound medicine on Aβ1–42 induced Alzheimer’s disease in rats
Folia Neuropathologica ( IF 1.5 ) Pub Date : 2021-01-11 , DOI: 10.5114/fn.2020.102439 Maiwulanijiang Yizibula 1, 2 , Zulifeiya Wusiman 1 , Nueramina Abudouhalike 1 , Batuer Maimaitiming 2
Folia Neuropathologica ( IF 1.5 ) Pub Date : 2021-01-11 , DOI: 10.5114/fn.2020.102439 Maiwulanijiang Yizibula 1, 2 , Zulifeiya Wusiman 1 , Nueramina Abudouhalike 1 , Batuer Maimaitiming 2
Affiliation
Introduction
We aimed to explore the role of a novel traditional herbal compound medicine (HCM) in Alzheimer’s disease (AD).
Material and methods
72 rats were randomized into control, AD, Donepezil and HCM groups. Injection of -amyloid peptide (A1–42) into the lateral ventricle was used to induce AD in rats. Rats in treatment groups received HCM (1.5, 3.0 and 6.0 g/kg) and Donepezil (0.92 mg/kg) for 21 days, respectively. The spatial learning and memory ability were observed by Morris water maze (MWM) test. Haematoxylin-eosin (HE) staining was carried out for pathological morphology. The contents of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the hippocampus were determined using the spectrophotometric method. A expression was measured by immunohistochemistry and Western blotting.
Results
Rats in HCM groups spent less time to locate the platform and performed better in spatial learning and memory than the AD group (p < 0.05). Hippocampus in the HCM (6.0 g/kg) group had a complete pyramidal cell layer, in which the structure of morphology was normal and the number of neurons was larger than in the AD group (p < 0.01). The contents of SOD, CAT, GSH-Px were notably increased and MDA content was significantly decreased in the hippocampus in HCM groups than in the AD group (p < 0.01). The expression levels of A1–42 in HCM groups were markedly decreased than in the AD group (p < 0.01).
Conclusions
HCM has a protective effect on the learning and memory capacity in AD in rats, indicating that HCM had cognitive enhancing potentials on AD.
中文翻译：
中草药复方对Aβ1-42诱导的阿尔茨海默病大鼠认知增强及神经保护作用
简介
我们旨在探索一种新型传统草药复合药物 (HCM) 在阿尔茨海默病 (AD) 中的作用。
材料与方法
72只大鼠随机分为对照组、AD组、多奈哌齐组和HCM组。将-淀粉样肽（A1-42）注射到侧脑室用于诱导大鼠AD。治疗组的大鼠分别接受 HCM（1.5、3.0 和 6.0 g/kg）和多奈哌齐（0.92 mg/kg）21 天。通过Morris水迷宫（MWM）测试观察空间学习和记忆能力。进行苏木精-伊红（HE）染色进行病理形态学。采用分光光度法测定海马中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）的含量。A 表达通过免疫组织化学和蛋白质印迹法测量。
结果
与 AD 组相比，HCM 组大鼠定位平台的时间更少，空间学习和记忆表现更好（p < 0.05）。HCM（6.0 g/kg）组海马具有完整的锥体细胞层，其形态结构正常，神经元数量多于AD组（p < 0.01）。与AD组相比，HCM组海马SOD、CAT、GSH-Px含量显着升高，MDA含量显着降低（p < 0.01）。HCM组A1-42的表达水平明显低于AD组（p < 0.01）。
结论
HCM对AD大鼠学习记忆能力有保护作用，说明HCM对AD具有认知增强潜能。
更新日期：2021-01-12
We aimed to explore the role of a novel traditional herbal compound medicine (HCM) in Alzheimer’s disease (AD).
Material and methods
72 rats were randomized into control, AD, Donepezil and HCM groups. Injection of -amyloid peptide (A1–42) into the lateral ventricle was used to induce AD in rats. Rats in treatment groups received HCM (1.5, 3.0 and 6.0 g/kg) and Donepezil (0.92 mg/kg) for 21 days, respectively. The spatial learning and memory ability were observed by Morris water maze (MWM) test. Haematoxylin-eosin (HE) staining was carried out for pathological morphology. The contents of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the hippocampus were determined using the spectrophotometric method. A expression was measured by immunohistochemistry and Western blotting.
Results
Rats in HCM groups spent less time to locate the platform and performed better in spatial learning and memory than the AD group (p < 0.05). Hippocampus in the HCM (6.0 g/kg) group had a complete pyramidal cell layer, in which the structure of morphology was normal and the number of neurons was larger than in the AD group (p < 0.01). The contents of SOD, CAT, GSH-Px were notably increased and MDA content was significantly decreased in the hippocampus in HCM groups than in the AD group (p < 0.01). The expression levels of A1–42 in HCM groups were markedly decreased than in the AD group (p < 0.01).
Conclusions
HCM has a protective effect on the learning and memory capacity in AD in rats, indicating that HCM had cognitive enhancing potentials on AD.
中文翻译：
中草药复方对Aβ1-42诱导的阿尔茨海默病大鼠认知增强及神经保护作用
简介
我们旨在探索一种新型传统草药复合药物 (HCM) 在阿尔茨海默病 (AD) 中的作用。
材料与方法
72只大鼠随机分为对照组、AD组、多奈哌齐组和HCM组。将-淀粉样肽（A1-42）注射到侧脑室用于诱导大鼠AD。治疗组的大鼠分别接受 HCM（1.5、3.0 和 6.0 g/kg）和多奈哌齐（0.92 mg/kg）21 天。通过Morris水迷宫（MWM）测试观察空间学习和记忆能力。进行苏木精-伊红（HE）染色进行病理形态学。采用分光光度法测定海马中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）的含量。A 表达通过免疫组织化学和蛋白质印迹法测量。
结果
与 AD 组相比，HCM 组大鼠定位平台的时间更少，空间学习和记忆表现更好（p < 0.05）。HCM（6.0 g/kg）组海马具有完整的锥体细胞层，其形态结构正常，神经元数量多于AD组（p < 0.01）。与AD组相比，HCM组海马SOD、CAT、GSH-Px含量显着升高，MDA含量显着降低（p < 0.01）。HCM组A1-42的表达水平明显低于AD组（p < 0.01）。
结论
HCM对AD大鼠学习记忆能力有保护作用，说明HCM对AD具有认知增强潜能。
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