Photoexcited Quantum Dots as Efficacious and Nontoxic Antibiotics in an Animal Model,ACS Biomaterials Science & Engineering

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Photoexcited Quantum Dots as Efficacious and Nontoxic Antibiotics in an Animal Model
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-01-12 , DOI: 10.1021/acsbiomaterials.0c01406
Colleen R. McCollum ₁ , Max Levy _{1,

2} , John R. Bertram _{2,

3} , Prashant Nagpal _{1,

2,

3,

4,

5} , Anushree Chatterjee _{1,

4,

5}

Affiliation

Drug-resistant bacterial infections are a growing cause of illness and death globally. Current methods of treatment are not only proving less effective but also perpetuate evolution of new resistance. Here we propose, through an in vivo model, a new treatment for drug-resistant bacterial infection that uses semiconductor nanoparticles, called quantum dots (QDs), that can be activated by light to produce superoxide to specifically and effectively kill drug-resistant bacteria. We adapt this technology for in vivo assessment of toxicity and treatment of a subcutaneous infection in mice. As our cadmium telluride QDs with 2.4 eV band gap (CdTe-2.4 QDs) are activated by blue light, we engineered LED patches to adhere to the infection site on mice, thus providing the light necessary for the activity of injected QDs and treatment of the infection. We show, through assessment of body weight, histology, and inflammation and oxidative stress markers in serum, that the CdTe-2.4 QDs are nontoxic at concentrations that reduce drug-resistant bacterial viability in subcutaneous abscesses. Further, CdTe-2.4 QDs did not accumulate in the body and were safely excreted in urine via renal clearance. CdTe-2.4 QD treatment decreased abscess viability by as much as 7 orders of magnitude. We thus propose an alternative treatment approach for drug-resistant topical infections: the injection of a low concentration of QDs and the application of an adhesive patch comprising only an LED and a battery. This treatment could revolutionize last-resort treatments of burn wounds, cellulitis, and other skin infections.

中文翻译：

光激发量子点作为一种有效和无毒的抗生素在动物模型中。

耐药细菌感染是全球疾病和死亡的增长原因。当前的治疗方法不仅证明效果较差，而且还会持续产生新的抗药性。在这里，我们提出通过体内模型，对一种耐药细菌感染的新治疗方法，该方法使用称为量子点（QDs）的半导体纳米颗粒，该纳米颗粒可以被光激活以产生超氧化物来特异性和有效地杀死耐药细菌。我们将这项技术应用于体内评估毒性和治疗小鼠皮下感染。由于我们的带隙为2.4 eV的碲化镉量子点（CdTe-2.4 QD）被蓝光激活，因此我们设计了LED贴片以粘附在小鼠的感染部位，从而为注射的量子点的活动和治疗提供了必要的光。感染。通过评估体重，组织学，血清中的炎症和氧化应激标志物，我们发现CdTe-2.4 QD在降低皮下脓肿中耐药细菌生存力的浓度下是无毒的。此外，CdTe-2.4 QD不会在体内积聚，而是通过肾脏清除被安全地排泄到尿液中。CdTe-2.4 QD处理可将脓肿的生存能力降低多达7个数量级。因此，我们提出了一种针对耐药性局部感染的替代治疗方法：注入低浓度的QD并应用仅包含LED和电池的胶粘剂贴片。这种治疗方法可能会彻底改变烧伤，蜂窝织炎和其他皮肤感染的最后治疗方法。

更新日期：2021-01-12

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