Abstract

Some designed macromolecules may be suitable substitutes for damaged tissue. This study aimed to use a novel Carbon Quantum Dots (CQD)-Alginate-Gelatin nanocomposite (NC) in the form of the microcapsule and as a scaffold for differentiation of mesenchymal stem cells into bone tissue. The CQD nanoparticles (NPs) were synthesized by the hydrothermal method and were characterized by FTIR, zeta potential, and DLS analysis. Alginate was used as a porous and flexible basis for the proper nutrition of the cells and keeping them safe from the body's immune system. To improve the biodegradability of CQD-alginate NC, gelatin protein was added to the NC. The CQD was employed at the concentration of 100 μg/ml and after placing the cells in the NC, microcapsules were made using the microdroplet technique. The binding and proliferation of mesenchymal stem cells (MSCs) in microcapsules were examined by DAPI staining and MTT. Also, bone differentiation of proliferated MSCs was investigated by evaluating the activity of alkaline phosphatase. The results showed that Alginate-Gelatin NC with its unique structure has an effective role in MSCs osteogenesis. Consequently, Alginate-Gelatin NC has been a perfect base for the performance of CQD NPs, so that, CQD-Alginate-Gelatin NC osteogenesis is higher than that of Alginate-Gelatin (CQD-free) composites after 14 days.

摘要

一些设计的大分子可能是受损组织的合适替代品。这项研究旨在以微胶囊的形式使用新型碳量子点（CQD）-海藻酸盐-明胶纳米复合材料（NC），并作为将间充质干细胞分化为骨组织的支架。通过水热法合成了CQD纳米颗粒（NPs），并通过FTIR，ζ电位和DLS分析对其进行了表征。海藻酸盐被用作细胞的适当营养的多孔且柔性的基础，并使其免受人体免疫系统的侵害。为了提高CQD-藻酸盐NC的生物降解能力，将明胶蛋白添加到NC中。CQD的浓度为100μg/ ml，将细胞置于NC中后，使用微滴技术制备微囊。通过DAPI染色和MTT检测微胶囊中的间充质干细胞（MSCs）的结合和增殖。另外，通过评估碱性磷酸酶的活性研究了增殖的MSC的骨分化。结果表明，藻酸盐-明胶NC具有独特的结构，在MSC的成骨中具有有效的作用。因此，藻酸盐-明胶NC是CQD NP性能的理想基础，因此14天后CQD-藻酸盐-明胶NC的成骨率高于藻酸盐-明胶（不含CQD）复合材料。结果表明，藻酸盐-明胶NC具有独特的结构，在MSC的成骨中具有有效的作用。因此，藻酸盐-明胶NC是CQD NP性能的理想基础，因此14天后CQD-藻酸盐-明胶NC的成骨率高于藻酸盐-明胶（不含CQD）复合材料。结果表明，藻酸盐-明胶NC具有独特的结构，在MSC的成骨中具有有效的作用。因此，藻酸盐-明胶NC是CQD NP性能的理想基础，因此14天后CQD-藻酸盐-明胶NC的成骨率高于藻酸盐-明胶（不含CQD）复合材料。