Abstract

Purpose

Radiation exposure of crickets during their 4^th juvenile molt inflicted ionizing radiation damage and altered growth rate, adult size at sexual maturity. High levels of ionizing radiation also impacted the subsequent generation, likely via heritable epigenetic mechanisms. Using radiation as a proxy for external stress, we aim to to understand transgenerational impacts of stress on non-irradiated offspring. Methods and Materials: We assess impacts of ionizing radiation on maturation mass and growth rate in F0 male and female house crickets (Acheta domesticus). We also assessed trans-generational impacts of irradiation on growth rate and maturation mass on non-irradiated offspring of irradiated parents compared to non-irradiated controls. Results: Early-life exposure to high levels of ionizing radiation induced lower growth rate and maturation mass compared to controls (p < 0.0001). Non-irradiated male F1 offspring of irradiated parents demonstrated significantly lower mass at maturation (P = 0.0012) and significantly faster time of maturation (p < 0.0001) compared to F1 non-irradiated controls. Conclusion: Our results show that a single early-life exposure to ionizing radiation can alter male offspring development through accelerated maturation and reduced maturation mass.

中文翻译：

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辐射诱发板球A（Acheta domesticus）的压力和世代相传的影响

摘要

目的

4在^第4^个少年蜕皮中的辐射暴露造成电离辐射损伤，并改变了性成熟时成年后的生长速度，成年大小。高水平的电离辐射也可能通过可遗传的表观遗传机制影响了后代。使用辐射作为外部压力的代名词，我们旨在了解压力对未辐射后代的跨代影响。方法和材料：我们评估电离辐射对F0雄性和雌性家house（Acheta domesticus）的成熟质量和生长速率的影响。我们还评估了辐照对未辐照父母的未辐照后代的生长速度和成熟质量的跨代影响，与未辐照对照相比。结果：与对照相比，生命早期暴露于高水平的电离辐射导致较低的生长速率和成熟质量（p <0.0001）。与未辐照的F1对照相比，未辐照的父母的未辐照雄性F1子代成熟时质量显着降低（P = 0.0012），成熟时间明显更快（p <0.0001）。结论：我们的结果表明，在生命的早期暴露于电离辐射中可以通过加速成熟和降低成熟质量来改变雄性后代的发育。