High‐throughput sequencing (HTS) is increasingly being used for the characterization and monitoring of biodiversity. If applied in a structured way, across broad geographical scales, it offers the potential for a much deeper understanding of global biodiversity through the integration of massive quantities of molecular inventory data generated independently at local, regional and global scales. The universality, reliability and efficiency of HTS data can potentially facilitate the seamless linking of data among species assemblages from different sites, at different hierarchical levels of diversity, for any taxonomic group and regardless of prior taxonomic knowledge. However, collective international efforts are required to optimally exploit the potential of site‐based HTS data for global integration and synthesis, efforts that at present are limited to the microbial domain. To contribute to the development of an analogous strategy for the nonmicrobial terrestrial domain, an international symposium entitled “Next Generation Biodiversity Monitoring” was held in November 2019 in Nicosia (Cyprus). The symposium brought together evolutionary geneticists, ecologists and biodiversity scientists involved in diverse regional and global initiatives using HTS as a core tool for biodiversity assessment. In this review, we summarize the consensus that emerged from the 3‐day symposium. We converged on the opinion that an effective terrestrial Genomic Observatories network for global biodiversity integration and synthesis should be spatially led and strategically united under the umbrella of the metabarcoding approach. Subsequently, we outline an HTS‐based strategy to collectively build an integrative framework for site‐based biodiversity data generation.

中文翻译：

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连接高通量生物多样性清单：用于全球整合和合成的基于位点的基因组框架的机会


高通量测序（HTS）越来越多地用于生物多样性的表征和监测。如果以结构化的方式在广泛的地理范围内应用，它可以通过整合在地方、区域和全球范围内独立生成的大量分子清单数据，为更深入地了解全球生物多样性提供了潜力。 HTS 数据的普遍性、可靠性和效率有可能促进来自不同地点、不同多样性层次、任何分类群的物种组合之间数据的无缝链接，无论先前的分类学知识如何。然而，需要国际社会共同努力，以最佳方式利用基于站点的高温超导数据的潜力进行全球整合和合成，目前的努力仅限于微生物领域。为了促进制定非微生物陆地领域的类似战略，题为“下一代生物多样性监测”的国际研讨会于 2019 年 11 月在尼科西亚（塞浦路斯）举行。此次研讨会汇集了参与使用 HTS 作为生物多样性评估核心工具的不同区域和全球倡议的进化遗传学家、生态学家和生物多样性科学家。在这篇综述中，我们总结了为期三天的研讨会所达成的共识。我们一致认为，用于全球生物多样性整合和综合的有效陆地基因组观测站网络应该在元条形码方法的保护下进行空间领导和战略统一。随后，我们概述了基于 HTS 的策略，以共同构建基于站点的生物多样性数据生成的综合框架。